4 results on '"Ippolito, Elio"'
Search Results
2. Reduced Follicular Regulatory T Cells in Spleen and Pancreatic Lymph Nodes of Patients With Type 1 Diabetes.
- Author
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Vecchione, Andrea, Jofra, Tatiana, Gerosa, Jolanda, Shankwitz, Kimberly, Di Fonte, Roberta, Galvani, Giuseppe, Ippolito, Elio, Cicalese, Maria Pia, Schultz, Andrew R., Seay, Howie R., Favellato, Mariagrazia, Milardi, Giulia, Stabilini, Angela, Ragogna, Francesca, Grogan, Pauline, Bianconi, Eleonora, Laurenzi, Andrea, Caretto, Amelia, Nano, Rita, and Melzi, Raffaela
- Subjects
REGULATORY T cells ,TYPE 1 diabetes ,LYMPH nodes ,SPLEEN ,LABORATORY mice ,AUTOIMMUNE diseases ,PANCREAS ,RESEARCH ,CELL culture ,ANIMAL experimentation ,RESEARCH methodology ,CASE-control method ,EVALUATION research ,COMPARATIVE studies ,RESEARCH funding ,LYMPHOCYTE count ,MICE - Abstract
In the attempt to understand the origin of autoantibody (AAb) production in patients with and at risk for type 1 diabetes (T1D), multiple studies have analyzed and reported alterations in T follicular helper (Tfh) cells in presymptomatic AAb+ subjects and patients with T1D. Yet, whether the regulatory counterpart of Tfh cells, represented by T follicular regulatory (Tfr) cells, is similarly altered is still unclear. To address this question, we performed analyses in peripheral blood, spleen, and pancreatic lymph nodes (PLN) of organ donor subjects with T1D. Blood analyses were also performed in living AAb- and AAb+ subjects. While negligible differences in the frequency and phenotype of blood Tfr cells were observed among T1D, AAb-, and AAb+ adult subjects, the frequency of Tfr cells was significantly reduced in spleen and PLN of T1D as compared with nondiabetic control subjects. Furthermore, adoptive transfer of Tfr cells delayed disease development in a mouse model of T1D, a finding that could indicate that Tfr cells play an important role in peripheral tolerance and regulation of autoreactive Tfh cells. Together, our findings provide evidence of Tfr cell alterations within disease-relevant tissues in patients with T1D, suggesting a role for Tfr cells in defective humoral tolerance and disease pathogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
3. Reduced Follicular Regulatory T Cells in Spleen and Pancreatic Lymph Nodes of Patients With Type 1 Diabetes
- Author
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Andrea Vecchione, Maria Pia Cicalese, Mariagrazia Favellato, Raffaela Melzi, Constantinos Petrovas, Elio Ippolito, Giuseppe Galvani, Kimberly Shankwitz, Eleonora Bianconi, Alessandro Aiuti, Angela Stabilini, Nichole Danzl, Manuela Battaglia, Roberta Di Fonte, Lorenzo Piemonti, Georgia Fousteri, Francesca Ragogna, Pauline Grogan, Emanuele Bosi, Rita Nano, Andrea Laurenzi, Todd M. Brusko, Jolanda Gerosa, Giulia Milardi, Tatiana Jofra, Howie R Seay, Amelia Caretto, Andrew R Schultz, Vecchione, Andrea, Jofra, Tatiana, Gerosa, Jolanda, Shankwitz, Kimberly, Di Fonte, Roberta, Galvani, Giuseppe, Ippolito, Elio, Cicalese, Maria Pia, Schultz, Andrew R, Seay, Howie R, Favellato, Mariagrazia, Milardi, Giulia, Stabilini, Angela, Ragogna, Francesca, Grogan, Pauline, Bianconi, Eleonora, Laurenzi, Andrea, Caretto, Amelia, Nano, Rita, Melzi, Raffaela, Danzl, Nichole, Bosi, Emanuele, Piemonti, Lorenzo, Aiuti, Alessandro, Brusko, Todd, Petrovas, Constantino, Battaglia, Manuela, and Fousteri, Georgia
- Subjects
Adult ,Adoptive cell transfer ,endocrine system ,Endocrinology, Diabetes and Metabolism ,Cell ,Spleen ,Mice, SCID ,T-Lymphocytes, Regulatory ,Pancreatic Lymph Node ,Mice ,Mice, Inbred NOD ,Follicular phase ,Internal Medicine ,medicine ,Animals ,Humans ,Lymphocyte Count ,Pancreas ,Cells, Cultured ,Type 1 diabetes ,business.industry ,Autoantibody ,Peripheral tolerance ,medicine.disease ,Mice, Inbred C57BL ,medicine.anatomical_structure ,Diabetes Mellitus, Type 1 ,Case-Control Studies ,Immunology ,Lymph Nodes ,Immunology and Transplantation ,business - Abstract
In the attempt to understand the origin of autoantibody (AAb) production in patients with and at-risk for T1D, multiple studies have analyzed and reported alterations in follicular helper T cells (Tfh) in presymptomatic AAb-positive subjects and patients with T1D. Yet, it is still not clear whether the regulatory counterpart of Tfh cells, represented by follicular regulatory T cells (Tfr), is similarly altered. To address this question, we performed analyses in peripheral blood, spleen and pancreatic lymph nodes (PLN) of organ donor subjects with T1D. Blood analyses were also performed in living AAb-negative and -positive subjects. While negligible differences in the frequency and phenotype of blood Tfr cells were observed between T1D, AAb-negative and AAb-positive adult subjects, the frequency of Tfr cells was significantly reduced in spleen and PLN of T1D as compared to non-diabetic controls. Furthermore, adoptive transfer of Tfr cells delayed disease development in a mouse model of T1D, a finding that could indicate that Tfr cells play an important role in peripheral tolerance and regulation of autoreactive Tfh cells. Together, our findings provide evidence of Tfr cell alterations within disease-relevant tissues in patients with T1D suggesting a role for Tfr cells in defective humoral tolerance and disease pathogenesis.
- Published
- 2021
4. Reduced PD-1 expression on circulating follicular and conventional FOXP3+ Treg cells in children with new onset type 1 diabetes and autoantibody-positive at-risk children.
- Author
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Vecchione, Andrea, Di Fonte, Roberta, Gerosa, Jolanda, Jofra, Tatiana, Cicalese, Maria Pia, Napoleone, Vincenzo, Ippolito, Elio, Galvani, Giuseppe, Ragogna, Francesca, Stabilini, Angela, Bianconi, Eleonora, Grogan, Pauline, Bonura, Clara, Bonfanti, Riccardo, Frontino, Giulio, Nano, Rita, Melzi, Raffaela, De Pellegrin, Maurizio, Laurenzi, Andrea, and Meschi, Franco
- Subjects
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TYPE 1 diabetes , *TRACHOMA , *BLOOD cells , *DESMOGLEINS , *INTERLEUKIN-7 , *T cells , *CELLS , *SUPPRESSOR cells - Abstract
Autoantibodies (AAbs) are a hallmark of Type 1 diabetes (T1D). Alterations in the frequency and phenotype of follicular helper (Tfh) T cells have been previously documented in patients with type 1 diabetes (T1D), but the contribution of follicular regulatory T (Treg) cells, which are responsible for suppressing AAb development, is less clear. Here, we investigated the frequency and activation status of follicular (CXCR5+) and conventional (CXCR5−) Treg cells in the blood of children with new-onset T1D, and children with risk for developing T1D (AAb-positive) and compared them to AAb-negative controls. Blood follicular and conventional Treg cells were higher in frequency in children with new onset T1D, but expressed reduced amounts of PD-1 as compared to AAb-negative children. Interestingly, the proportion of circulating FOXP3+ Tregs expressing PD-1 was also reduced in AAb-positive at-risk children as compared to AAb-negative controls, suggesting its potential use as a biomarker of disease progression. Follicular Treg cells were reduced in frequency in the spleens of prediabetic NOD mice as they became older and turned diabetic. Interestingly, PD-1 expression declined also on circulating follicular and conventional Treg cells in prediabetic NOD mice as they aged. Together, these findings show that the frequency of circulating follicular and conventional Treg cells and their levels of PD-1 change with disease progression in children at-risk for developing T1D and in NOD mice. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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