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4 results on '"Marcelo Cordeiro-Dos-Santos"'

1. Immunologic Biomarkers in Peripheral Blood of Persons With Tuberculosis and Advanced HIV

Author

Artur T. L. Queiroz, Mariana Araújo-Pereira, Beatriz Barreto-Duarte, Adriano Gomes-Silva, Allyson G. Costa, Alice M. S. Andrade, João Pedro Miguez-Pinto, Renata Spener-Gomes, Alexandra B. Souza, Aline Benjamin, Flavia Sant’Anna, Marina C. Figueiredo, Vidya Mave, Padmini Salgame, Jerrold J. Ellner, Timothy R. Sterling, Marcelo Cordeiro-dos-Santos, Bruno B. Andrade, and Valeria C. Rolla

Subjects
biomarkers, diagnosis, tuberculosis, advanced HIV, IL-15, IL-10, Immunologic diseases. Allergy, RC581-607
Abstract

IntroductionTuberculosis (TB) is a common opportunistic infection among people living with HIV. Diagnostic tests such as culture, Xpert-MTB-RIF, and ULTRA have low sensitivity in paucibacillary TB disease; a blood biomarker could improve TB diagnostic capabilities. We assessed soluble factors to identify biomarkers associated with TB among persons with advanced HIV.MethodsA case-control (1:1) study was conducted, with participants from Rio de Janeiro and Manaus, Brazil. People living with HIV presenting with CD4 count ≤100 cells/mm3 were eligible to participate. Cases had culture-confirmed TB (N=15) (positive for Mycobacterium tuberculosis [Mtb]); controls had HIV-infection only (N=15). Study visits included baseline, month 2 and end of TB therapy, during which samples of peripheral blood were obtained. A panel containing 29 biomarkers including cytokines, chemokines and growth factors was utilized to assess candidate biomarkers using Luminex technology in cryopreserved EDTA plasma samples. We used neural network analysis, based on machine learning, to identify biomarkers (single or in combination) that best distinguished cases from controls. Additional multi-dimensional analyses provided detailed profiling of the systemic inflammatory environment in cases and controls.ResultsMedian CD4 count and HIV-1 RNA load values were similar between groups at all timepoints. Persons with TB had lower body mass index (BMI) (median=19.6, Interquartile Range [IQR]=18.6-22.3) than controls (23.7; IQR: 21.8 = 25.5, p=0.004). TB coinfection was also associated with increased frequency of other comorbidities. The overall profile of plasma cytokines, chemokines and growth factors were distinct between the study groups at all timepoints. Plasma concentrations of IL-15 and IL-10 were on average lower in TB cases than in controls. When used in combination, such markers were able to discriminate between TB cases and controls with the highest degree of accuracy at each study timepoint.ConclusionAmong persons with advanced HIV, plasma concentrations of IL-15 and IL-10 can be used in combination to identify TB disease regardless of time on anti-TB treatment.

Published
2022
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2. A summary of the proceedings of a meeting on the treatment of latent tuberculosis infection in target populations in Brazil

Author

Valeria Rolla, Anete Trajman, Masae Kawamura, Solange Cavalcante, Elizabeth Soares, Filipe de Barros Perini, Anna Cristina Calçada Carvalho, Julio Croda, Jose Roberto Lapa e Silva, Carolina Arana Stanis Schmaltz, Flavia Marinho Sant’Anna, Mayara Bastos, Adriano Gomes, Betina Durovni, Valeria Saraceni, Elisangela Silva, Fernanda Carvalho de Queiroz Mello, Marcelo Cordeiro dos Santos, Marcia Pinto, Marisa Santos, Fernando Augusto Dias e Sanches, and Sandra Maria do Valle Leone de Oliveira

Subjects
Diseases of the respiratory system, Health Services Needs and Demand, RC705-779, business.industry, Latent Tuberculosis, Medicine, Humans, Tuberculosis, Mycobacterium tuberculosis, business, Letter to the Editor, Brazil, Microbiology
Published
2020

3. Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study

Author

Isabela Neves de Almeida, Thiago da Silva Santos Malaquias, Wânia da Silva Carvalho, Julio Croda, Fernanda Carvalho de Queiroz Mello, João Paulo Amaral Hadaad, Jaciara Diniz, Maria de Fátima Filardi Oliveira Mansur, Reginalda Ferreira de Melo Medeiros, Fátima Cristina Onofre Fandinho Montes, Erica Chimara, Maria Laura Halon, Alexandra Brito, Diana Machado, Pedro Almeida da Silva, Maria C.S. Lourenço, Andrea von Groll, Maria Luiza Lopes, Miguel Viveiros, Juliana Maira Watanabe Pinhata, Afrânio Lineu Kritski, Marcelo Cordeiro dos Santos, Rosângela Siqueira Oliveira, Silvana Spíndola de Miranda, Lida Jouca de Assis Figueredo, and Maria Lucia Rosa Rossetti

Subjects
0106 biological sciences, Tuberculosis, nitrate reductase, Drug resistance, Resist?ncia a M?ltiplos Medicamentos, 01 natural sciences, Mycobacterium tuberculosis, Estudo Multic?ntrico, Nitrato Redutase / farmacologia, 010608 biotechnology, MDR, medicine, Mycobacterium / efeitos dos f?rmacos, Ethambutol, Multidisciplinary, biology, business.industry, Isoniazid, Sensibilidade e Especificidade, Tuberculose / diagn?stico, medicine.disease, biology.organism_classification, bacterial infections and mycoses, Virology, Löwenstein–Jensen medium, tuberculosis, Streptomycin, technology, business, TP248.13-248.65, Rifampicin, Biotechnology, medicine.drug
Abstract

This research was funded by MINAS GERAIS STATE RESEARCH SUPPORT FOUNDATION (FAPEMIG), grants numbers 65/10 and CDS-APQ-03266-13, and by NATIONAL COUNCIL FOR SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENT (CNPQ) grants numbers 310174/2014-7 and 446796/2014-0. Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil. Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil. Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil. Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil. Federal University of Minas Gerais. Faculty of Pharmacy. Department of Social Pharmacy. Belo Horizonte, MG, Brazil. Federal University of Minas Gerais. Veterinary School. Department of Preventive Veterinary Medicine. Belo Horizonte, MG, Brazil. Federal University of Rio Grande. Faculty of Medicine. Laboratory of Mycobacteria. Rio Grande, RS, Brazil. Federal University of Rio Grande. Faculty of Medicine. Laboratory of Mycobacteria. Rio Grande, RS, Brazil. Federal University of Rio Grande. Faculty of Medicine. Laboratory of Mycobacteria. Rio Grande, RS, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazil. Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazil. Federal University of Rio de Janeiro. Institute of Chest Diseases. Clementino Fraga Filho University Hospital. Rio de Janeiro, RJ, Brazil. Federal University of Rio de Janeiro. Institute of Chest Diseases. Clementino Fraga Filho University Hospital. Rio de Janeiro, RJ, Brazil. Federal University of Grande Dourados. Faculty of Health Sciences. Dourados, MS, Brazil / Oswaldo Cruz Foundation. Campo Grande, Mato Grosso do Sul, MS, Brazil. Adolfo Lutz Institute. Bacteriology Center. Tuberculosis and Mycobacteriosis Center. S?o Paulo, SP, Brazil. Adolfo Lutz Institute. Bacteriology Center. Tuberculosis and Mycobacteriosis Center. S?o Paulo, SP, Brazil. Adolfo Lutz Institute. Bacteriology Center. Tuberculosis and Mycobacteriosis Center. S?o Paulo, SP, Brazil. State Secretariat of Health of Rio Grande do Sul. State Center for Health Surveillance. Center for Scientific and Technological Development. Porto Alegre, RS, Brazil. State Secretariat of Health of Rio Grande do Sul. State Center for Health Surveillance. Center for Scientific and Technological Development. Porto Alegre, RS, Brazil. Oswaldo Cruz Foundation. National Institute of Infectology Evandro Chagas. Laboratory of Bacteriology and Bioassays of Rio de Janeiro. Rio de Janeiro, RJ, Brazil. Sergio Arouca National Public Health School. Professor H?lio Fraga Reference Center. Rio de Janeiro, RJ, Brazil. Sergio Arouca National Public Health School. Professor H?lio Fraga Reference Center. Rio de Janeiro, RJ, Brazil. Nova University of Lisbon. Institute of Hygiene and Tropical Medicine. Medical Microbiology Unit, Global Health and Tropical Medicine. Lisboa, Portugal. Nova University of Lisbon. Institute of Hygiene and Tropical Medicine. Medical Microbiology Unit, Global Health and Tropical Medicine. Lisboa, Portugal. Federal University of Rio de Janeiro. Faculty of Medicine. Tuberculosis Research Center. Rio de Janeiro, RJ, Brazil. Background: The Commercial Kit SIRE Nitratase? PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.

Published
2020

4. Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study

Author

Silvana Spindola de Miranda, Isabela Neves de Almeida, Maria de Fátima Filardi Oliveira Mansur, Lida Jouca de Assis Figueredo, Wânia da Silva Carvalho, João Paulo Amaral Hadaad, Jaciara de Lourdes do Carmo Guimarães Diniz, Andrea von Groll, Pedro Almeida da Silva, Maria Luiza Lopes, Marcelo Cordeiro dos Santos, Alexandra Brito, Fernanda Carvalho de Queiroz Mello, Thiago da Silva Santos Malaquias, Julio Croda, Juliana Maira Watanabe Pinhata, Rosângela Siqueira de Oliveira, Erica Chimara, Maria Lúcia Rossetti, Maria Laura Halon, Maria Cristina Lourenço, Reginalda Ferreira de Melo Medeiros, Fátima Cristina Onofre Fandinho Montes, Diana Machado, Miguel Viveiros, and Afrânio Lineu Kritski

Subjects
tuberculosis, MDR, nitrate reductase, technology, Biotechnology, TP248.13-248.65
Abstract

Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.

Published
2020
Full Text
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