1. Cardiomyocyte oxidants production may signal to T. cruzi intracellular development.
- Author
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Dias PP, Capila RF, do Couto NF, Estrada D, Gadelha FR, Radi R, Piacenza L, and Andrade LO
- Subjects
- Animals, Antioxidants pharmacology, Calcium metabolism, Cell Respiration, Cells, Cultured, Chagas Cardiomyopathy parasitology, Chagas Disease parasitology, Cytokines biosynthesis, Cytokines immunology, Humans, Hydrogen Peroxide metabolism, Hydrogen Peroxide pharmacology, Mice, Mice, Inbred BALB C, Myocytes, Cardiac drug effects, Oxidants pharmacology, Oxidative Stress, Reactive Oxygen Species metabolism, Superoxides metabolism, Trypanosoma cruzi classification, Trypanosoma cruzi physiology, Host-Parasite Interactions, Myocytes, Cardiac metabolism, Myocytes, Cardiac parasitology, Oxidants metabolism, Trypanosoma cruzi growth & development
- Abstract
Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, presents a variable clinical course, varying from asymptomatic to serious debilitating pathologies with cardiac, digestive or cardio-digestive impairment. Previous studies using two clonal T. cruzi populations, Col1.7G2 (T. cruzi I) and JG (T. cruzi II) demonstrated that there was a differential tissue distribution of these parasites during infection in BALB/c mice, with predominance of JG in the heart. To date little is known about the mechanisms that determine this tissue selection. Upon infection, host cells respond producing several factors, such as reactive oxygen species (ROS), cytokines, among others. Herein and in agreement with previous data from the literature we show that JG presents a higher intracellular multiplication rate when compared to Col1.7G2. We also showed that upon infection cardiomyocytes in culture may increase the production of oxidative species and its levels are higher in cultures infected with JG, which expresses lower levels of antioxidant enzymes. Interestingly, inhibition of oxidative stress severely interferes with the intracellular multiplication rate of JG. Additionally, upon H2O2-treatment increase in intracellular Ca2+ and oxidants were observed only in JG epimastigotes. Data presented herein suggests that JG and Col1.7G2 may sense extracellular oxidants in a distinct manner, which would then interfere differently with their intracellular development in cardiomyocytes.
- Published
- 2017
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