1. Trypanosoma brucei: four tandemly linked genes for fatty acyl-CoA synthetases.
- Author
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Jiang DW, Ingersoll R, Myler PJ, and Englund PT
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Blotting, Southern, Cloning, Molecular, Coenzyme A Ligases chemistry, DNA, Protozoan chemistry, Gene Expression Regulation, Enzymologic, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Coenzyme A Ligases genetics, Trypanosoma brucei brucei enzymology, Trypanosoma brucei brucei genetics
- Abstract
We have cloned four acyl CoA synthetase (ACS) genes from Trypanosoma brucei strain 927. Each of these genes encodes a polypeptide about 78 kDa in size and all four contain the "ACS signature motif." Sequence alignments indicate that these proteins are 46%-95% identical in amino acid sequence. Interestingly, three of them share almost identical C-termini (about 215 amino acid residues). Southern blots suggest that these genes are present in a single copy, and Northern blots reveal that all four are expressed in both bloodstream and procyclic trypanosomes., (Copyright 2000 Academic Press.)
- Published
- 2000
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