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Your search keyword '"Renato Simões Moreira"' showing total 2 results
Start Over Author "Renato Simões Moreira" Topic trypanosoma
2 results on '"Renato Simões Moreira"'

1. First record of Trypanosoma evansi DNA in Dichelacera alcicornis and Dichelacera januarii (Diptera: Tabanidae) flies in South America

Author

Carlos José Raupp Ramos, Cintia de Souza Franco, Sabrina Pogere da Luz, Júlia Marques, Ketriane Mota de Souza, Luiz Flávio Nepomuceno do Nascimento, Gabriella Bassi das Neves, Renato Simões Moreira, and Luiz Claudio Miletti

Subjects
Tabanidae, Vectors, Transmission, Polymerase chain reaction, Trypanosoma, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Trypanosoma evansi infects a large number of wild and domestic animals and causes a spoliative disease known as surra. It is mechanically transmitted, mainly by biting flies of the genera Tabanus and Stomoxys. The detection of T. evansi DNA in the feeding apparatus of Dichelacera alcicornis and Dichelacera januarii from South America is reported, to the best of our knowledge, for the first time. Methods Tabanids were collected weekly from February 2018 to February 2019 from two sites. The feeding apparatus was removed and DNA extraction, polymerase chain reaction and sequencing were performed. Results A 205-base pair fragment of the variant surface protein RoTat 1.2 gene, confirmed by DNA sequencing, was amplified from the feeding apparatus of D. alcicornis and D. januarii. Conclusions This is, to the best of our knowledge, the first record of T. evansi DNA in South American tabanids. Graphical abstract

Published
2023
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2. Serum proteomic signature of Trypanosoma evansi –infected mice for identification of potential biomarkers

Author

Franciane Batista, Leonardo Antonio Fernandes, Glauber Wagner, Nathália Anderson Calomeno, Luiz Claudio Miletti, Renato Simões Moreira, and Júlia Marques

Subjects
Proteomics, Trypanosoma, General Veterinary, biology, Protozoan Proteins, Computational Biology, General Medicine, Trypanosoma evansi, biology.organism_classification, Surra, Blood proteins, Epitope, Serology, Hsp70, Microbiology, Mice, Trypanosomiasis, Animals, Epitopes, B-Lymphocyte, Parasitology, Amino Acid Sequence, Biomarkers
Abstract

Trypanosoma evansi is the agent of "surra," a trypanosomosis endemic in many areas worldwide. Trypanosoma proteins released/secreted during infection are attractive biomarkers for disease detection and monitoring. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we performed a comprehensive analysis of the serum proteome of mice infected with T.evansi and detected changes in the abundance of parasite and host serum proteins during infection. Following bioinformatics analysis, 30 T. evansi proteins were identified in the mice serum including known targets such as pyruvate kinase 1, β-tubulin, actin A, heat shock protein 70, and cyclophilin A. We also identified two exclusive VSG epitopes which are novel putative biomarker targets. In addition, upregulation of 31 mouse proteins, including chitinase-like protein 3 and monocyte differentiation antigen CD14, were observed. Identification of parasite-specific biomarkers in the host serum is critical for the development of reliable serological/ assays for differential diagnosis.

Published
2021
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