1. Acid heteropolysaccharides with potent antileishmanial effects.
- Author
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Kangussu-Marcolino MM, do Rosário MM, Noseda MD, Duarte ME, Ducatti DR, Cassolato JE, Iacomini M, Martinez GR, Rocha ME, Cadena SM, and Noleto GR
- Subjects
- Animals, Cytotoxicity, Immunologic drug effects, Leishmania metabolism, Macrophages, Peritoneal drug effects, Macrophages, Peritoneal immunology, Macrophages, Peritoneal metabolism, Macrophages, Peritoneal parasitology, Mice, Nitric Oxide biosynthesis, Parasitic Sensitivity Tests, Superoxides metabolism, Leishmania drug effects, Polysaccharides chemistry, Polysaccharides pharmacology, Trypanocidal Agents chemistry, Trypanocidal Agents pharmacology
- Abstract
The present study investigated in vitro the effects of sulphated heterorhamnan (Go3), iota-/nu-carrageenans (G3d and EHW-I) and arabinogalactan (ARAGAL) polysaccharides on macrophage activation and inhibition of intracellular amastigotes of Leishmania (L.) amazonensis. All the sulphated polysaccharides (Go3, G3d and EHW-I) promoted increased nitric oxide production varying from 71 to 110%. The leishmanicidal activity of all compounds was compared to the inhibition effect of Meglumine Antimoniate at 300μg/mL (∼79%), used as positive control. Inhibition of Leishmania (L.) amazonensis growth was 55% with 5μg/mL of Go3, 50% and 98% to G3d and EHW-I, respectively at 10μg/mL, and 88% with 10μg/mL of ARAGAL. The superoxide anion scavenging activity for the sulphated polysaccharides varied from approximately 30-55% at 10μg/mL. In conclusion, the results of the present study indicate the promising potential of these polysaccharides for the development of new alternative therapeutic agents against leishmaniasis., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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