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Your search keyword '"M, Urbancíková"' showing total 4 results
Start Over Author "M, Urbancíková" Topic tropomyosin
4 results on '"M, Urbancíková"'

1. Using tropomyosin polymorphic phenomenon for detection of differences between rat spontaneous tumor cells and their in vitro oncovirus supertransformats.

Author

Urbancíková M, Grófová M, and Veselý P

Subjects
Animals, Avian Sarcoma Viruses, Cytoskeleton analysis, Mammary Neoplasms, Experimental analysis, Neoplasms, Experimental pathology, Rats, Tropomyosin immunology, Cell Transformation, Neoplastic metabolism, Neoplasms, Experimental analysis, Tropomyosin analysis, Tumor Virus Infections analysis
Abstract

Biochemical and immunological comparative studies of rat tumor cells of spontaneous origin and in vitro supertransformed cell populations have been done. We have focused on characterization of differences in tropomyosin molecular forms in the individual cell populations. Our experiments have shown that differences of tropomyosin forms exist not only between spontaneous transformants and supertransformants but also between supertransformants and normal rat fibroblasts. It means that superinfection of spontaneous transformants by avian sarcoma virus B77 have induced changes in tropomyosin synthesis but a pattern of tropomyosin forms in super-transformants has not been equal or similar to that of normal fibroblasts.

Published
1984

2. Quantitative changes of tropomyosin synthesis in RSV-transformed mammalian cells in comparison with normal fibroblasts.

Author

Urbancíková M and Grófová M

Subjects
Animals, Avian Sarcoma Viruses, Cell Line, Fibroblasts metabolism, Mice, Mice, Inbred C57BL, Molecular Weight, Rats, Rats, Inbred Strains, Cell Transformation, Neoplastic, Sarcoma, Avian metabolism, Tropomyosin biosynthesis
Abstract

Protein profiles of three tumor mammalian cell lines and three control cell lines were analyzed by two-dimensional gel electrophoresis. Comparison of the cells labeled metabolically with 35S-methionine has revealed obvious differences in an area of molecular weights 34 000--36 000 daltons and pH 4.5-5.0. Two spots have been proposed to be alpha- and beta-tropomyosin subunits. Their synthesis decreases after cell transformation.

Published
1982

3. Immunofluorescent detection of actin cytoskeleton in spontaneously transformed and B77-supertransformed cells using antibody to tropomyosin

Author

M, Urbancíková and M, Grófová

Subjects
Immune Sera, Fluorescent Antibody Technique, Mammary Neoplasms, Experimental, Tropomyosin, Fibroblasts, Actins, Cell Line, Rats, Actin Cytoskeleton, Cell Transformation, Neoplastic, Avian Sarcoma Viruses, Rats, Inbred Lew, Animals, Cells, Cultured, Cytoskeleton
Abstract

Differences in immunofluorescence of actin cytoskeleton between normal rat fibroblasts and two transformed cell lines SAMIV and SAMB77 were detected by antiserum to tropomyosin. In both transformed cell lines reduction in number and shortening of microfilament bundles (stress fibers) were observed. In some transformed cells ruffling membranes (peripheral ruffles) were seen. Differences were found in actin cytoskeleton among individual cells of spontaneous transformants (SAMIV) and supertransformants (SAMB77).

Published
1990

4. Using tropomyosin polymorphic phenomenon for detection of differences between rat spontaneous tumor cells and their in vitro oncovirus supertransformats

Author

M, Urbancíková, M, Grófová, and P, Veselý

Subjects
Tumor Virus Infections, Cell Transformation, Neoplastic, Avian Sarcoma Viruses, Animals, Mammary Neoplasms, Experimental, Neoplasms, Experimental, Tropomyosin, Cytoskeleton, Rats
Abstract

Biochemical and immunological comparative studies of rat tumor cells of spontaneous origin and in vitro supertransformed cell populations have been done. We have focused on characterization of differences in tropomyosin molecular forms in the individual cell populations. Our experiments have shown that differences of tropomyosin forms exist not only between spontaneous transformants and supertransformants but also between supertransformants and normal rat fibroblasts. It means that superinfection of spontaneous transformants by avian sarcoma virus B77 have induced changes in tropomyosin synthesis but a pattern of tropomyosin forms in super-transformants has not been equal or similar to that of normal fibroblasts.

Published
1984

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