Search

Your search keyword '"DE AMBROGIO E."' showing total 3 results
Start Over Author "DE AMBROGIO E." Topic triticum
3 results on '"DE AMBROGIO E."'

1. Searching for wheat plants with low toxicity in celiac disease: Between direct toxicity and immunologic activation.

Author

Carroccio A, Di Prima L, Noto D, Fayer F, Ambrosiano G, Villanacci V, Lammers K, Lafiandra D, De Ambrogio E, Di Fede G, Iacono G, and Pogna N

Subjects
Antibodies metabolism, Celiac Disease immunology, Celiac Disease pathology, Gene Deletion, Humans, Interferon-gamma metabolism, Interleukin-10 metabolism, Interleukin-2 metabolism, Intestinal Mucosa immunology, Intestinal Mucosa pathology, Prolamins immunology, Tissue Culture Techniques, Triticum genetics, Triticum immunology, Celiac Disease metabolism, Intestinal Mucosa metabolism, Prolamins toxicity, Triticum toxicity
Abstract

Background: Natural or induced variations in the noxiousness of gluten proteins for celiac disease (CD) patients are currently being investigated for their potential in breeding wheat crops with reduced toxicity., Aims: We evaluated the bread wheat line C173 for its effects on the in vitro-grown duodenal mucosa of CD patients., Methods: In vitro-grown duodenal mucosa biopsies of 19 CD patients on a gluten-free diet were exposed to peptic/tryptic-digested prolamins from bread wheat line C173 lacking gliadin-glutenin subunits, analyzed for morphology, cytokine and anti-tTG antibody production, and compared with mucosa biopsies exposed to prolamins from wild-type cv. San Pastore., Results: Duodenal mucosa biopsies exposed to prolamins from C173 and San Pastore released higher amounts of IFN-γ, IL-2, IL-10 and anti-tTG antibodies in the culture medium than untreated controls. The line C173 differed from cv. San Pastore as it did not produce negative effects on enterocyte height, suggesting that manipulating prolamin composition can affect innate immune responses of CD mucosa to wheat gluten., Conclusions: Our data demonstrated that this gliadin-deficient wheat has a lower direct toxicity but activates an immunologic reaction of the duodenal mucosa like that of the common wheat species., (Copyright © 2010 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.)

Published
2011
Full Text
View/download PDF

2. Wheat deficient in gliadins: promising tool for treatment of coeliac disease.

Author

Frisoni M, Corazza GR, Lafiandra D, De Ambrogio E, Filipponi C, Bonvicini F, Borasio E, Porceddu E, and Gasbarrini G

Subjects
Adult, Celiac Disease metabolism, Celiac Disease pathology, Digestion, Duodenum metabolism, Duodenum pathology, Humans, Intestinal Mucosa metabolism, Intestinal Mucosa pathology, Organ Culture Techniques, Celiac Disease diet therapy, Gliadin chemistry, Triticum chemistry
Abstract

The toxicity of two varieties of bread wheat, one poor in alpha and beta gliadins and the other poor in alpha, beta, gamma, and omega gliadins, has been tested. The peptic-tryptic digest of these wheats was assessed using coeliac mucosa in an in vitro organ culture system. A significantly lower toxicity was found in respect of bread wheat containing all gliadin fractions. These results suggest new opportunities for the treatment of coeliac disease.

Published
1995
Full Text
View/download PDF

3. Searching for wheat plants with low toxicity in celiac disease: Between direct toxicity and immunologic activation

Author

Francesca Fayer, Lidia Di Prima, Gaetana Di Fede, Vincenzo Villanacci, Norberto Pogna, Giuseppe Ambrosiano, Giuseppe Iacono, Antonio Carroccio, Davide Noto, Enzo De Ambrogio, Domenico Lafiandra, Karen M. Lammers, and Carroccio A, Di Prima L, Noto D, Fayer F, Ambrosiano G, Villanacci V, Lammers K, Lafiandra D, De Ambrogio E, Di Fede G, Iacono G, Pogna N.

Subjects
Settore MED/09 - Medicina Interna, Enterocyte, medicine.medical_treatment, Antibodies, Tissue Culture Techniques, Immunologic activation, Interferon-gamma, medicine, Humans, Intestinal Mucosa, Prolamin, Common wheat, Triticum, Hepatology, biology, Gastroenterology, food and beverages, toxicity, immunologic activation, Interleukin-10, Cytokine, medicine.anatomical_structure, Anti-transglutaminase antibodies, Immunology, Toxicity, biology.protein, Interleukin-2, Antibody, Gene Deletion, wheat plant, celiac disease, Prolamins
Abstract

Background Natural or induced variations in the noxiousness of gluten proteins for celiac disease (CD) patients are currently being investigated for their potential in breeding wheat crops with reduced toxicity. Aims We evaluated the bread wheat line C173 for its effects on the in vitro -grown duodenal mucosa of CD patients. Methods In vitro -grown duodenal mucosa biopsies of 19 CD patients on a gluten-free diet were exposed to peptic/tryptic-digested prolamins from bread wheat line C173 lacking gliadin–glutenin subunits, analyzed for morphology, cytokine and anti-tTG antibody production, and compared with mucosa biopsies exposed to prolamins from wild-type cv. San Pastore. Results Duodenal mucosa biopsies exposed to prolamins from C173 and San Pastore released higher amounts of IFN-γ, IL-2, IL-10 and anti-tTG antibodies in the culture medium than untreated controls. The line C173 differed from cv. San Pastore as it did not produce negative effects on enterocyte height, suggesting that manipulating prolamin composition can affect innate immune responses of CD mucosa to wheat gluten. Conclusions Our data demonstrated that this gliadin-deficient wheat has a lower direct toxicity but activates an immunologic reaction of the duodenal mucosa like that of the common wheat species.

Published
2011

Catalog

Books, media, physical & digital resources
See catalog results