1. Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation
- Author
-
Anli Yang, Huadi Chen, Jianwei Lin, Ming Han, Xiaopeng Yuan, Tao Zhang, Qingwei Nian, Mengran Peng, Dian Li, Chenglin Wu, and Xiaoshun He
- Subjects
Male ,Principal Component Analysis ,Transplantation ,RNA, Untranslated ,diagnosis ,fungal infection ,Bioengineering ,non-coding rnas ,General Medicine ,Applied Microbiology and Biotechnology ,Mice, Inbred C57BL ,Disease Models, Animal ,Immunocompromised Host ,Mice ,Mycoses ,Animals ,Humans ,TP248.13-248.65 ,Biotechnology - Abstract
The occurrence of fungal infection seriously affects the survival and life quality of transplanted patients. The accurate diagnosis is of particular importance in the early stage of infection. To develop a novel diagnostic method for this kind of patient, we established a post-transplant immunosuppressed mice model with fungus inoculation and collected their peripheral blood at specific time points after infection. After screening by microarray, differentially expressed miRNAs and lncRNAs were selected and homologously analyzed with those of human beings from the gene database. These miRNAs and lncRNAs candidates were validated by qRT-PCR in peripheral blood samples from transplanted patients. We found that, compared with normal transplanted patients, the levels of miR-215 and miR-let-7 c were up-regulated in the plasma of patients with fungal infection (P
- Published
- 2022