1. PKM2 对肝内胆管癌细胞增殖及上皮间质化的影响.
- Author
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周 闯, 陆 旭, 宋盛平, 于 龙, 叶建文, and 翟文龙
- Abstract
Objective To investigate the expression of pyruvate kinase M2 ( PKM2) in intrahepatic cholangiocarcinoma (ICC) and the effect of PKM2 on the proliferation and epithelial-to -mesenchymal transition (EMT) of ICC cells. Methods PKM2 expression was evaluated in ICC tissues and cell lines by Real-time quantitative reverse-transcription polymerase chain reaction ( RT-PCR), and immunohistochemistry assays. In vilro, we knocked down PKM2 expression in ICC cell lines and investigated the biological function and the underlying mechanism of PKM2 in ICC. Results RT-PCR results showed that PKM2 was highly expressed in ICC ( P·<0. 05). Moreover. PKM2 knockdown inhibited cell proliferation and the invasive capacities of ICC cells. and inhibited Wnt/β-Catenin signaling. which subsequently regulated EMT signaling pathway in vitro. Conclusion PKM2 expression in cholangiocarcinoma is significantly higher than that in adjacent tissues. PKM2 can regulate EMT and invasion and metastasis of ICC via the Wnt/β-Catenin signal. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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