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Watermelon ( Citrullus lanatus L.), which is an economically important cucurbit crop that is cultivated worldwide, is vulnerable to various adverse environmental conditions. Small heat shock protein 20s (HSP20s) are the most abundant plant HSPs and they play important roles in various biotic and abiotic stress responses. However, they have not been systematically investigated in watermelon. In this study, we identified 44 watermelon HSP20 genes and analyzed their gene structures, conserved domains, phylogenetic relationships, chromosomal distributions, and expression profiles. All of the watermelon HSP20 proteins have a conserved the α-crystallin (ACD) domain. Half of the ClHSP20s arose through gene duplication events. Plant HSP20s were grouped into 18 subfamiles and a new subfamily, nucleo-cytoplasmic XIII (CXIII), was identified in this study. Numerous stress- and hormone-responsive cis -elements were detected in the putative promoter regions of the watermelon HSP20 genes. Different from that in other species, half of the watermelon HSP20s were repressed by heat stress. Plant HSP20s displayed diverse responses to different virus infections and most of the ClHSP20s were generally repressed by Cucumber green mottle mosaic virus (CGMMV). Some ClHSP20s exhibited similar transcriptional responses to abscisic acid, melatonin, and CGMMV. Subcellular localization analyses of six selected HSP20- green fluorescence protein fusion proteins revealed diverse subcellular targeting. Some ClHSP20 proteins were affected by CGMMV, as reflected by changes in the size, number, and distribution of fluorescent granules. These systematic analyses provide a foundation for elucidating the physiological functions and biological roles of the watermelon HSP20 gene family.

Sponge gourd belongs to the Cucurbitaceae family and Luffa genus. It is an economically valuable vegetable crop with medicinal properties. The fruit size of sponge gourd presents distinct diversity; however, the molecular insights of fruit size regulation remain uncharacterized. Therefore, two sponge gourd materials with distinct fruit sizes were selected for a comparative transcriptome analysis. A total of 1390 genes were detected as differentially expressed between long sponge gourd (LSG) and short sponge gourd (SSG) samples, with 885 downregulated and 505 upregulated in SSG compared with LSG. KEGG pathway enrichment analysis revealed that the MAPK signaling pathway, biosynthesis of secondary metabolites, and plant hormone signal transduction were significantly enriched. The DEGs involved in the cell cycle and cell division, plant hormone metabolism, and MAPK signal transduction were crucial for sponge gourd fruit size regulation. Additionally, the transcription factor families of ERF, NAC, bHLH, MYB, WRKY, and MADS-box were associated with fruit size regulation. The qRT-PCR validation for selected DEGs were generally consistent with the RNA-Seq results. These results obtained the candidate genes and pathways associated with fruit size and lay the foundation for revealing the molecular mechanisms of fruit size regulation in sponge gourd. [ABSTRACT FROM AUTHOR]

Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus, which cause diseases in cucurbits, especially watermelon. In watermelon, symptoms develop on the whole plant, including leaves, stems, peduncles, and fruit. To better understand the molecular mechanisms of watermelon early responses to CGMMV infection, a comparative transcriptome analysis of 24 h CGMMV-infected and mock-inoculated watermelon leaves was performed. A total of 1641 differently expressed genes (DEGs) were identified, with 886 DEGs upregulated and 755 DEGs downregulated after CGMMV infection. A functional analysis indicated that the DEGs were involved in photosynthesis, plant–pathogen interactions, secondary metabolism, and plant hormone signal transduction. In addition, a few transcription factor families, including WRKY, MYB, HLH, bZIP and NAC, were responsive to the CGMMV-induced stress. To confirm the high-throughput sequencing results, 15 DEGs were validated by qRT-PCR analysis. The results provide insights into the identification of candidate genes or pathways involved in the responses of watermelon leaves to CGMMV infection. [ABSTRACT FROM AUTHOR]

The color of radish (Raphanus sativus) taproot skin is an important visual quality. 'Xinlimei' radish is a red-fleshed cultivar with skin that changes color from red to white and finally to green at the mature stage, and appearance quality is strongly affected if the red color does not fade completely on a single taproot or simultaneously among different taproots. In the present study, anthocyanin and chlorophyll contents and the transcriptome of radish taproot skin at three distinct coloration stages were analyzed to explore the mechanism of color changes. The results showed that decreased anthocyanin and increased chlorophyll contents correlated with the color-fading process. Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially expressed genes indicated that anthocyanin and chlorophyll metabolism pathways play important roles in color changes. In red color-fading process, the expression levels of anthocyanin biosynthetic genes (except PAL and C4H), a transport gene (RsTT19), and two anthocyanin biosynthesis transcription factors (TFs), RsMYB1 and RsTT8 , were significantly downregulated, whereas peroxidase-encoding genes were significantly upregulated. In the skin-greening process, expression of most chlorophyll biosynthetic genes and two TFs (RsGLK1 and RsGLK2) that likely positively regulate chlorophyll biosynthesis was significantly upregulated. Thus, changes in the expression of these genes may be responsible for the color changes that occur in 'Xinlimei' taproot skin. This is the first report on the roles of chlorophyll metabolism genes and their dynamic relationship with anthocyanin metabolism genes in radish. The findings provide valuable information and theoretical guidelines for improving the appearance quality of 'Xinlimei' radish taproots. • Decreased anthocyanin and increased chlorophyll contents occur during color changes. • Anthocyanin biosynthesis, transport and degradation affect red color fading. • Chlorophyll metabolism related genes in radish are firstly identified. • Upregulation of chlorophyll metabolism genes are responsible for skin greening. [ABSTRACT FROM AUTHOR]