10 results on '"Westendorp, Bart"'
Search Results
2. E2f8 mediates tumor suppression in postnatal liver development
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Kent, Lindsey N., Rakijas, Jessica B., Pandit, Shusil K., Westendorp, Bart, Chen, Hui Zi, Huntington, Justin T., Tang, Xing, Bae, Sooin, Srivastava, Arunima, Senapati, Shantibhusan, Koivisto, Christopher, Martin, Chelsea K., Cuitino, Maria C., Perez, Miguel, Clouse, Julian M., Chokshi, Veda, Shinde, Neelam, Kladney, Raleigh, Sun, Daokun, Perez-Castro, Antonio, Matondo, Ramadhan B., Nantasanti, Sathidpak, Mokry, Michal, Huang, Kun, Machiraju, Raghu, Fernandez, Soledad, Rosol, Thomas J., Coppola, Vincenzo, Pohar, Kamal S., Pipas, James M., Schmidt, Carl R., De Bruin, Alain, Leone, Gustavo, LS Pathobiologie, dPB RMSC, LS Pathobiologie, and dPB RMSC
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Male ,0301 basic medicine ,Biopsy ,MOUSE ,E2F7 Transcription Factor ,HEPATOCELLULAR-CARCINOMA ,Gene expression ,Oligonucleotide Array Sequence Analysis ,Medicine(all) ,Liver Neoplasms ,PROLIFERATION ,General Medicine ,Null allele ,CANCER ,3. Good health ,Chromatin ,READ ALIGNMENT ,TRANSCRIPTION FACTORS ,Liver ,Female ,Research Article ,Protein Binding ,Signal Transduction ,Carcinoma, Hepatocellular ,GENES ,Genotype ,Cell Survival ,Repressor ,Biology ,03 medical and health sciences ,Protein Domains ,Journal Article ,medicine ,Animals ,Humans ,E2F ,Gene ,Transcription factor ,Alleles ,Cell Proliferation ,Sequence Analysis, RNA ,Cancer ,DNA ,medicine.disease ,CELL-CYCLE CONTROL ,Repressor Proteins ,MODEL ,MICE ,030104 developmental biology ,Immunology ,Hepatocytes ,Cancer research ,Gene Deletion - Abstract
E2F-mediated transcriptional repression of cell cycle-dependent gene expression is critical for the control of cellular proliferation, survival, and development. E2F signaling also interacts with transcriptional programs that are downstream of genetic predictors for cancer development, including hepatocellular carcinoma (HCC). Here, we evaluated the function of the atypical repressor genes E2f7 and E2f8 in adult liver physiology. Using several loss-of-function alleles in mice, we determined that combined deletion of E2f7 and E2f8 in hepatocytes leads to HCC. Temporal-specific ablation strategies revealed that E2f8's tumor suppressor role is critical during the first 2 weeks of life, which correspond to a highly proliferative stage of postnatal liver development. Disruption of E2F8's DNA binding activity phenocopied the effects of an E2f8 null allele and led to HCC. Finally, a profile of chromatin occupancy and gene expression in young and tumor-bearing mice identified a set of shared targets for E2F7 and E2F8 whose increased expression during early postnatal liver development is associated with HCC progression in mice. Increased expression of E2F8-specific target genes was also observed in human liver biopsies from HCC patients compared to healthy patients. In summary, these studies suggest that E2F8-mediated transcriptional repression is a critical tumor suppressor mechanism during postnatal liver development.
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- 2016
3. Mechanisms used by cancer cells to tolerate drug-induced replication stress.
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Segeren, Hendrika A. and Westendorp, Bart
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CANCER cells , *DNA replication , *DRUG side effects , *TRANSCRIPTION factors , *CHECKPOINT kinase 1 - Abstract
Activation of oncogenes in cancer cells forces cell proliferation, leading to DNA replication stress (RS). As a consequence, cancer cells heavily rely on the intra S-phase checkpoint for survival. This fundamental principle formed the basis for the development of inhibitors against key players of the intra S-phase checkpoint, ATR and CHK1. These drugs are often combined with chemotherapeutic drugs that interfere with DNA replication to exacerbate RS and exhaust the intra S-phase checkpoint in cancer cells. However, drug resistance impedes efficient clinical use, suggesting that some cancer cells tolerate severe RS. In this review, we describe how an increased nucleotide pool, boosted stabilization and repair of stalled forks and firing of dormant origins fortify the RS response in cancer cells. Notably, the vast majority of the genes that confer RS tolerance are regulated by the E2F and NRF2 transcription factors. These transcriptional programs are frequently activated in cancer cells, allowing simultaneous activation of multiple tolerance avenues. We propose that the E2F and NRF2 transcriptional programs can be used as biomarker to select patients for treatment with RS-inducing drugs and as novel targets to kill RS-tolerant cancer cells. Together, this review aims to provide a framework to maximally exploit RS as an Achilles' heel of cancer cells. [ABSTRACT FROM AUTHOR]
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- 2022
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4. E2F8 is essential for polyploidization in mammalian cells.
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Pandit, Shusil K., Westendorp, Bart, Nantasanti, Sathidpak, van Liere, Elsbeth, Tooten, Peter C. J., Cornelissen, Peter W. A., Toussaint, Mathilda J. M., Lamers, Wouter H., and de Bruin, Alain
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POLYPLOIDY , *MAMMALIAN cell cycle , *LIVER cells , *TRANSCRIPTION factors , *PROMOTERS (Genetics) - Abstract
Polyploidization is observed in all mammalian species and is a characteristic feature of hepatocytes, but its molecular mechanism and biological significance are unknown. Hepatocyte polyploidization in rodents occurs through incomplete cytokinesis, starts after weaning and increases with age. Here, we show in mice that atypical E2F8 is induced after weaning and required for hepatocyte binucleation and polyploidization. A deficiency in E2f8 led to an increase in the expression level of E2F target genes promoting cytokinesis and thereby preventing polyploidization. In contrast, loss of E2f1 enhanced polyploidization and suppressed the polyploidization defect of hepatocytes deficient for atypical E2Fs. In addition, E2F8 and E2F1 were found on the same subset of target promoters. Contrary to the long-standing hypothesis that polyploidization indicates terminal differentiation and senescence, we show that prevention of polyploidization through inactivation of atypical E2Fs has, surprisingly, no impact on liver differentiation, zonation, metabolism and regeneration. Together, these results identify E2F8 as a repressor and E2F1 as an activator of a transcriptional network controlling polyploidization in mammalian cells. [ABSTRACT FROM AUTHOR]
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- 2012
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5. E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1.
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Weijts, Bart G M W, Bakker, Walbert J, Cornelissen, Peter W A, Liang, Kuo-Hsuan, Schaftenaar, Frank H, Westendorp, Bart, de Wolf, Charlotte A C M T, Paciejewska, Maya, Scheele, Colinda L G J, Kent, Lindsey, Leone, Gustavo, Schulte-Merker, Stefan, and de Bruin, Alain
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TRANSCRIPTION factors ,NEOVASCULARIZATION ,CELL cycle regulation ,LABORATORY mice ,EMBRYOLOGY ,VASCULAR endothelial growth factors ,HYPOXIA-inducible factor 1 ,PROMOTERS (Genetics) - Abstract
The E2F family of transcription factors plays an important role in controlling cell-cycle progression. While this is their best-known function, we report here novel functions for the newest members of the E2F family, E2F7 and E2F8 (E2F7/8). We show that simultaneous deletion of E2F7/8 in zebrafish and mice leads to severe vascular defects during embryonic development. Using a panel of transgenic zebrafish with fluorescent-labelled blood vessels, we demonstrate that E2F7/8 are essential for proper formation of blood vessels. Despite their classification as transcriptional repressors, we provide evidence for a molecular mechanism through which E2F7/8 activate the transcription of the vascular endothelial growth factor A (VEGFA), a key factor in guiding angiogenesis. We show that E2F7/8 directly bind and stimulate the VEGFA promoter independent of canonical E2F binding elements. Instead, E2F7/8 form a transcriptional complex with the hypoxia inducible factor 1 (HIF1) to stimulate VEGFA promoter activity. These results uncover an unexpected link between E2F7/8 and the HIF1-VEGFA pathway providing a molecular mechanism by which E2F7/8 control angiogenesis. [ABSTRACT FROM AUTHOR]
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- 2012
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6. The E2F6 repressor activates gene expression in myocardium resulting in dilated cardiomyopathy.
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Westendorp, Bart, Major, Jennifer L., Nader, Moni, Salih, Maysoon, Leenen, Frans H. H., and Tuana, Balwant S.
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CONNEXINS , *MICRORNA , *TRANSCRIPTION factors , *TRANSGENIC mice , *GENE expression , *DILATED cardiomyopathy - Abstract
The E2F/Rb pathway regulates cardiac growth and development and holds great potential as a therapeutic target. The E2F6 repressor is a unique E2F member that acts independently of pocket proteins. Forced expression of E2F6 in mouse myocardium induced heart failure and mortality, with severity of symptoms correlating to E2F6 levels. Echocardiography demonstrated a 37% increase (P<0.05) in left ventricular end-diastolic diameter and reduced ejection fraction (<40%, P<0.05) in young transgenic (Tg) mice. Microarray and qPCR analysis revealed a paradoxical increase in E2F-responsive genes, which regulate the cell cycle, without changes in cardiomyocyte cell number or size in Tg mice. Young adult Tg mice displayed a 75% (P<0.01) decrease in gap junction protein connexin-43, resulting in abnormal electrocardiogram including a 24% (P<0.05) increase in PR interval. Further, mir-206, which targets connexin-43, was up-regulated 10-fold (P<0.05) in Tg myocardium. The mitogen-activated protein kinase pathway, which regulates the levels of miR-206 and connexin43, was activated in Tg hearts. Thus, deregulated E2F6 levels evoked abnormal gene expression at transcriptional and post-transcriptional levels, leading to cardiac remodeling and dilated cardiomyopathy. The data highlight an unprecedented role for the strict regulation of the E2F pathway in normal postnatal cardiac function. [ABSTRACT FROM AUTHOR]
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- 2012
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7. Atypical E2Fs either Counteract or Cooperate with RB during Tumorigenesis Depending on Tissue Context.
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Moreno, Eva, Pandit, Shusil K., Toussaint, Mathilda J. M., Bongiovanni, Laura, Harkema, Liesbeth, van Essen, Saskia C., van Liere, Elsbeth A., Westendorp, Bart, de Bruin, Alain, Indovina, Paola, Pentimalli, Francesca, and Giordano, Antonio
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DISEASE progression ,GENETIC mutation ,LIVER tumors ,ANIMAL experimentation ,NEOPLASTIC cell transformation ,GENE expression ,PITUITARY tumors ,TRANSCRIPTION factors ,CELL lines ,MICE - Abstract
Simple Summary: In virtually all human malignancies, the CDK-RB-E2F pathway is dysregulated resulting in the activation of the E2F transcriptional network. Rb and atypical E2Fs are the most important negative regulators of E2F-dependent transcription during tumorigenesis. However, it is unknown whether they cooporate or act independently in tumor development. Here we show that combined loss of RB and atypical E2Fs in mice enhances tumorigenesis in the liver, while in the pituitary gland, we observe inhibition of tumorigenesis. These findings suggest that the interaction between RB and atypical E2Fs in controlling tumorigenesis occurs in a tissue cell-type specific manner. E2F-transcription factors activate many genes involved in cell cycle progression, DNA repair, and apoptosis. Hence, E2F-dependent transcription must be tightly regulated to prevent tumorigenesis, and therefore metazoan cells possess multiple E2F regulation mechanisms. The best-known is the Retinoblastoma protein (RB), which is mutated in many cancers. Atypical E2Fs (E2F7 and −8) can repress E2F-target gene expression independently of RB and are rarely mutated in cancer. Therefore, they may act as emergency brakes in RB-mutated cells to suppress tumor growth. Currently, it is unknown if and how RB and atypical E2Fs functionally interact in vivo. Here, we demonstrate that mice with liver-specific combinatorial deletion of Rb and E2f7/8 have reduced life-spans compared to E2f7/8 or Rb deletion alone. This was associated with increased proliferation and enhanced malignant progression of liver tumors. Hence, atypical repressor E2Fs and RB cooperatively act as tumor suppressors in hepatocytes. In contrast, loss of either E2f7 or E2f8 largely prevented the formation of pituitary tumors in Rb
+/− mice. To test whether atypical E2Fs can also function as oncogenes independent of RB loss, we induced long-term overexpression of E2f7 or E2f8 in mice. E2F7 and −8 overexpression increased the incidence of tumors in the lungs, but not in other tissues. Collectively, these data show that atypical E2Fs can promote but also inhibit tumorigenesis depending on tissue type and RB status. We propose that the complex interactions between atypical E2Fs and RB on maintenance of genetic stability underlie this context-dependency. [ABSTRACT FROM AUTHOR]- Published
- 2021
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8. Expression of the cell cycle regulator E2F6 during cardiac development and left ventricular remodeling after myocardial infarction.
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Westendorp, Bart, Nader, Moni, Leenen, Frans H., and Tuana, Balwant S.
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CELL cycle regulation , *GENES , *VENTRICULAR remodeling , *TISSUES , *TRANSCRIPTION factors - Abstract
The mechanisms underlying the permanent cell cycle arrest of cardiomyocytes shortly after birth are incompletely understood, but likely involve alterations in expression of E2F-responsive genes. E2F6 is a repressor of E2F-responsive genes, and we observed high expression in the heart. To explore its function in cardiac growth, we studied E2F6 expression during normal cardiac development and left ventricular (LV) remodeling after myocardial infarction (MI). LV tissue was collected from rats at different stages of fetal and postnatal development. Expression of E2F6 and of the transcription factors E2F1 and 3 was assessed using real-time PCR and Western blot analysis. MI was induced by coronary artery ligation and LV tissue remote from the scar was collected 3 months post MI. Expression of E2F6 markedly increased during postnatal development while the expression of E2F3 and E2F1 was reduced in adults. Inducing quiescence in fetal cardiomyoblasts caused an upregulation of E2F6 protein (255+25%, P<0.05). After MI, E2F6 mRNA and protein decreased by ∼25% in non-infarcted LV (both P<0.05 versus sham) in an infarct-size dependent manner. Expression of E2F1 and 3 did not change after MI. In conclusion, E2F6 upregulation coincides with cell cycle arrest of cardiomyocytes. Remarkably, E2F6 was downregulated in non-infarcted LV post-MI, suggesting a role in cardiac cell growth and remodeling. Supported by an HSFO grant. [ABSTRACT FROM AUTHOR]
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- 2007
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9. Dosage-dependent copy number gains in E2f1 and E2f3 drive hepatocellular carcinoma.
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Kent, Lindsey N., Sooin Bae, Shih-Yin Tsai, Xing Tang, Srivastava, Arunima, Koivisto, Christopher, Martin, Chelsea K., Ridolfi, Elisa, Miller, Grace C., Zorko, Sarah M., Plevris, Emilia, Hadjiyannis, Yannis, Perez, Miguel, Nolan, Eric, Kladney, Raleigh, Westendorp, Bart, de Bruin, Alain, Fernandez, Soledad, Rosol, Thomas J., and Pohar, Kamal S.
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LIVER cancer , *DNA copy number variations , *TUMOR suppressor genes , *GENETIC mutation , *TRANSCRIPTION factors , *CANCER invasiveness - Abstract
Disruption of the retinoblastoma (RB) tumor suppressor pathway, either through genetic mutation of upstream regulatory components or mutation of RB1 itself, is believed to be a required event in cancer. However, genetic alterations in the RB-regulated E2F family of transcription factors are infrequent, casting doubt on a direct role for E2Fs in driving cancer. In this work, a mutation analysis of human cancer revealed subtle but impactful copy number gains in E2F1 and E2F3 in hepatocellular carcinoma (HCC). Using a series of loss- and gain-of-function alleles to dial E2F transcriptional output, we have shown that copy number gains in E2f1 or E2f3b resulted in dosage-dependent spontaneous HCC in mice without the involvement of additional organs. Conversely, germ-line loss of E2f1 or E2f3b, but not E2f3a, protected mice against HCC. Combinatorial mapping of chromatin occupancy and transcriptome profiling identified an E2F1- and E2F3B-driven transcriptional program that was associated with development and progression of HCC. These findings demonstrate a direct and cell-autonomous role for E2F activators in human cancer. [ABSTRACT FROM AUTHOR]
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- 2017
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10. E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration.
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Sladky, Valentina C., Knapp, Katja, Soratroi, Claudia, Heppke, Julia, Eichin, Felix, Rocamora-Reverte, Lourdes, Szabo, Tamas G., Bongiovanni, Laura, Westendorp, Bart, Moreno, Eva, van Liere, Elsbeth A., Bakker, Bjorn, Spierings, Diana C.J., Wardenaar, René, Pereyra, David, Starlinger, Patrick, Schultze, Simon, Trauner, Michael, Stojakovic, Tatjana, and Scharnagl, Hubert
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PLOIDY , *LIVER regeneration , *ANEUPLOIDY , *POLYPLOIDY , *TRANSCRIPTION factors , *P53 antioncogene , *LIVER cells - Abstract
E2F transcription factors control the cytokinesis machinery and thereby ploidy in hepatocytes. If or how these proteins limit proliferation of polyploid cells with extra centrosomes remains unknown. Here, we show that the PIDDosome, a signaling platform essential for caspase-2-activation, limits hepatocyte ploidy and is instructed by the E2F network to control p53 in the developing as well as regenerating liver. Casp2 and Pidd1 act as direct transcriptional targets of E2F1 and its antagonists, E2F7 and E2F8, that together co-regulate PIDDosome expression during juvenile liver growth and regeneration. Of note, whereas hepatocyte aneuploidy correlates with the basal ploidy state, the degree of aneuploidy itself is not limited by PIDDosome-dependent p53 activation. Finally, we provide evidence that the same signaling network is engaged to control ploidy in the human liver after resection. Our study defines the PIDDosome as a primary target to manipulate hepatocyte ploidy and proliferation rates in the regenerating liver. • The PIDDosome controls hepatocyte ploidy during postnatal development & regeneration • The PIDDosome defines the speed of liver regeneration posthepatectomy • Aneuploidy in the liver correlates with basal ploidy state but is not limited by CASP2 • E2F family members regulate expression of CASP2 and PIDD1 for liver ploidy control Sladky et al. report a key role for the PIDDosome in regulating p53 activation to limit hepatocyte polyploidy during juvenile liver growth and regeneration. Expression of essential PIDDosome components is controlled by a E2F-family regulated circuitry. The study defines the PIDDosome as a putative target to enhance liver regeneration. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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