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Your search keyword '"Oh, E.‐J."' showing total 2 results
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2 results on '"Oh, E.‐J."'

1. Application of anti-DFS70 antibody and specific autoantibody test algorithms to patients with the dense fine speckled pattern on HEp-2 cells.

Author

Lee H, Kim Y, Han K, and Oh EJ

Subjects
Autoantibodies immunology, Cell Line, Tumor, Connective Tissue Diseases diagnosis, Dermatomyositis diagnosis, Dermatomyositis immunology, Enzyme-Linked Immunosorbent Assay, Humans, Immunoassay, Lupus Erythematosus, Systemic diagnosis, Lupus Erythematosus, Systemic immunology, Mixed Connective Tissue Disease diagnosis, Mixed Connective Tissue Disease immunology, Rheumatic Diseases diagnosis, Rheumatic Diseases immunology, Scleroderma, Systemic diagnosis, Scleroderma, Systemic immunology, Sensitivity and Specificity, Sjogren's Syndrome diagnosis, Adaptor Proteins, Signal Transducing immunology, Algorithms, Antibodies, Antinuclear immunology, Connective Tissue Diseases immunology, Fluorescent Antibody Technique, Indirect methods, Sjogren's Syndrome immunology, Transcription Factors immunology
Abstract

Objectives: Whereas antinuclear antibodies (ANAs) detected by indirect immunofluorescence (IIF) have diagnostic significance, the dense fine speckled (DFS) pattern on HEp-2 cells may be an exclusionary marker for ANA-associated rheumatic disease (AARD). The aim of this study was to evaluate a new algorithm considering anti-DFS70 antibodies for routine ANA testing., Method: From ANA requested sequential 10 528 sera, 181 sera samples showing the DFS pattern were additionally tested for anti-DFS70 antibodies by an enzyme-linked immunosorbent assay (ELISA-DFS70) and for specific-ANAs. Specific-ANAs(+)/IIF-DFS(-) control sera samples (n = 50) were also tested., Results: Of the 181 IIF-DFS-positive sera samples, 82.9% (n = 150) were from non-AARD patients and 112 (61.9%) patients had non-rheumatic diseases (NRD), including the most common clinical feature of dermatitis (18.2%). The ELISA-DFS70 was positive in 109 (60.2%) sera and was negative in all control sera. Specific-ANAs were similarly detected as 25.7% (28/109) and 22.2% (16/72) of ELISA-DFS70(+) and ELISA-DFS70(+) patients, respectively (p > 0.05). The prevalence of non-AARD was 95.1% and 25.1% in the ELISA-DFS70(+)/specific-ANAs(-) and ELISA-DFS70(-)/specific-ANAs (+) groups, respectively., Conclusions: In patients with a HEp-2 DFS pattern, the additional ELISA-DFS70 and specific-ANAs test could improve the efficiency of diagnosing AARD. The detection of anti-DFS70 antibodies should be included in test algorithms for ANA testing.

Published
2016
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2. E2F-dependent transcription of the raf proto-oncogene during Drosophila development.

Author

Kwon EJ, Oh EJ, Kim YS, Hirose F, Ohno K, Nishida Y, Matsukage A, Yamaguchi M, and Yoo MA

Subjects
Animals, Base Sequence, Binding, Competitive, DNA Probes genetics, DNA Probes metabolism, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, E2F Transcription Factors, Embryo, Nonmammalian metabolism, Genes, Reporter genetics, In Situ Hybridization, Mutation genetics, Promoter Regions, Genetic genetics, Protein Binding, RNA, Messenger analysis, RNA, Messenger genetics, Recombinant Fusion Proteins metabolism, Response Elements genetics, Retinoblastoma-Binding Protein 1, Transcription Factors genetics, Transcriptional Activation, Carrier Proteins, Cell Cycle Proteins, Drosophila Proteins, Drosophila melanogaster embryology, Drosophila melanogaster genetics, Gene Expression Regulation, Developmental, Proto-Oncogene Proteins c-raf genetics, Trans-Activators, Transcription Factors metabolism, Transcription, Genetic genetics
Abstract

D-raf, a Drosophila homolog of the raf proto-oncogene, has diverse functions throughout development and is transcribed in a wide range of tissues, with high levels of expression in the ovary and in association with rapid proliferation. The expression pattern resembles those of S phase genes, which are regulated by E2F transcription factors. In the 5'-flanking region of D-raf, four sequences (E2F sites 1-4) similar to the E2F recognition sequence were found, one of them (E2F site 3) being recognized efficiently by Drosophila E2F (dE2F) in vitro. Transient luciferase expression assays confirmed activation of the D-raf gene promoter by dE2F/dDP. Expression of Draf-lacZ was greatly reduced in embryos homozygous for the dE2F mutation. These results suggest that dE2F is likely to be an important regulator of D-raf transcription.

Published
2001
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