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3 results on '"Endosomal Sorting Complexes Required for Transport immunology"'

1. Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells.

Author

Kumar B, Veettil MV, Roy A, and Chandran B

Subjects
Cell Culture Techniques methods, Cell Line, DNA-Binding Proteins immunology, Endosomal Sorting Complexes Required for Transport immunology, Endothelial Cells virology, Humans, Staining and Labeling methods, Transcription Factors immunology, Virus Internalization, Biological Assay methods, DNA-Binding Proteins metabolism, Endosomal Sorting Complexes Required for Transport metabolism, Endosomes metabolism, Herpesvirus 8, Human immunology, Protein Interaction Mapping methods, Transcription Factors metabolism
Abstract

Proximity ligation assay (PLA) is a newly developed technique that outperforms the traditional immunoassays for visualizing the in situ endogenous protein-protein interactions and localizations and the activation of proteins in cell culture systems as well as in tissue sections. PLA, when combined with cellular marker staining, becomes a powerful approach to identify differential interaction of the proteins of endosomal sorting complex required for transport (ESCRT) at distinct stages of virus infection. In this chapter, we describe a PLA protocol to study the localization and interaction between the ESCRT protein TSG101 and endosomal markers in early stages of viral endocytosis in in vitro infected cells.

Published
2019
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2. TSG101: Tumor Susceptibility Gene 101 (tsg101) Product-Role in Therapy Against HIV/AIDS.

Author

Qadir MI and Zafar M

Subjects
Acquired Immunodeficiency Syndrome immunology, Acquired Immunodeficiency Syndrome virology, Anti-HIV Agents immunology, Antibodies, Monoclonal immunology, DNA-Binding Proteins antagonists & inhibitors, DNA-Binding Proteins therapeutic use, Endosomal Sorting Complexes Required for Transport antagonists & inhibitors, Endosomal Sorting Complexes Required for Transport therapeutic use, HIV Infections drug therapy, HIV Infections immunology, HIV Infections virology, Humans, Transcription Factors antagonists & inhibitors, Transcription Factors therapeutic use, Acquired Immunodeficiency Syndrome drug therapy, Anti-HIV Agents therapeutic use, Antibodies, Monoclonal therapeutic use, DNA-Binding Proteins immunology, Endosomal Sorting Complexes Required for Transport immunology, Transcription Factors immunology
Abstract

HIV infection presents a major community health hazard, partially because the HIV virus is capable of evading antiretroviral therapies. Most anti-HIV drugs were intended to target virus-encoded mechanisms; however, some host-encoded molecules comparatively execute a vital role in the life cycle of virus. Thus, these might be considered as target sites for antiviral agents. TSG101 is important among these antiviral therapies because, as a cytoplasmic molecule, it facilitates viral budding and release. In this review, HIV-infected cells have TSG101 on their surface and thus can be used in antibody-based therapies. The development of a monoclonal antibody CB8-2 lessens the assembly of viruses from infected cells. This mechanism represents the potential use of TSG101-directed antibodies to fight against AIDS.

Published
2017
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3. Essential role of ubiquitin and TSG101 protein in formation and function of the central supramolecular activation cluster.

Author

Vardhana S, Choudhuri K, Varma R, and Dustin ML

Subjects
Animals, B-Lymphocytes immunology, B7-1 Antigen immunology, DNA-Binding Proteins genetics, Down-Regulation, Endosomal Sorting Complexes Required for Transport genetics, Humans, Immunological Synapses metabolism, Mice, Phosphorylation, RNA, Small Interfering genetics, Receptors, Antigen, T-Cell metabolism, Signal Transduction, T-Lymphocytes metabolism, Transcription Factors genetics, DNA-Binding Proteins immunology, DNA-Binding Proteins metabolism, Endosomal Sorting Complexes Required for Transport immunology, Endosomal Sorting Complexes Required for Transport metabolism, Immunological Synapses immunology, Receptors, Antigen, T-Cell immunology, T-Lymphocytes immunology, Transcription Factors immunology, Transcription Factors metabolism, Ubiquitin metabolism
Abstract

Agonist MHC-peptide complexes in the immunological synapse (IS) signal through T cell receptor (TCR) microclusters (MCs) that converge into a central supramolecular activation cluster (cSMAC). The determinants and function of the cSMAC remain unknown. We demonstrate an essential role for ubiquitin (Ub) and TSG101, but less so for HRS, in signal processing events at the cSMAC. Using siRNA in primary T cells, we show that Ub recognition by TSG101 is required for cSMAC formation, TCR MC signal termination, TCR downregulation, and segregation of TCR-MHC-peptide from PKC-theta-enriched signaling complexes. Weak agonist MHC-peptide induced CD80-dependent TCR MCs that dissociated in the center of the IS without recruiting TSG101. These results support TSG101-dependent recognition of CD80-independent TCR MCs as a molecular checkpoint for TCR downregulation., (Copyright 2010 Elsevier Inc. All rights reserved.)

Published
2010
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