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Early retinal progenitor cells (RPCs) in vertebrates produce lineages that vary greatly both in terms of cell number and fate composition, yet how this variability is achieved remains unknown. One possibility is that these RPCs are individually distinct and that each gives rise to a unique lineage. Another is that stochastic mechanisms play upon the determinative machinery of equipotent early RPCs to drive clonal variability. Here we show that a simple model, based on the independent firing of key fate-influencing transcription factors, can quantitatively account for the intrinsic clonal variance in the zebrafish retina and predict the distributions of neuronal cell types in clones where one or more of these fates are made unavailable., (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)

During development, the lymphatic vasculature forms as a second network derived chiefly from blood vessels. The transdifferentiation of embryonic venous endothelial cells (VECs) into lymphatic endothelial cells (LECs) is a key step in this process. Specification, differentiation and maintenance of LEC fate are all driven by the transcription factor Prox1, yet the downstream mechanisms remain to be elucidated. We here present a single‐cell transcriptomic atlas of lymphangiogenesis in zebrafish, revealing new markers and hallmarks of LEC differentiation over four developmental stages. We further profile single‐cell transcriptomic and chromatin accessibility changes in zygotic prox1a mutants that are undergoing a LEC‐VEC fate shift. Using maternal and zygotic prox1a/prox1b mutants, we determine the earliest transcriptomic changes directed by Prox1 during LEC specification. This work altogether reveals new downstream targets and regulatory regions of the genome controlled by Prox1 and presents evidence that Prox1 specifies LEC fate primarily by limiting blood vascular and haematopoietic fate. This extensive single‐cell resource provides new mechanistic insights into the enigmatic role of Prox1 and the control of LEC differentiation in development. Synopsis: Transdifferentiation of embryonic venous endothelial cells (VECs) into lymphatic endothelial cells (LECs) has not been transcriptionally profiled in vivo. Here, extensive profiling of lymphangiogenesis in wild‐type and mutant zebrafish uncovers how the earliest specification and differentiation events are regulated. Single‐cell RNA‐seq at four key stages of development identifies hallmarks of lymphatic differentiation in the zebrafish embryo.Single‐nuclei ATAC‐seq analysis identifies specific enhancers and regulatory elements in different endothelial cell types during development.The transcription factor Prox1 is needed to regulate chromatin state during LEC fate maintenance and to balance blood and lymphatic vascular gene expression.During the earliest stages of lymphatic specification, Prox1 primarily downregulates blood vascular and haematopoietic transcriptional programs.Notch signalling components are expressed following LEC specification and Notch1b controls lymphatic development. [ABSTRACT FROM AUTHOR]

Summary: The adult zebrafish brain, unlike mammalian counterparts, can regenerate after injury owing to the neurogenic capacity of stem cells with radial glial character. We hypothesized that injury-induced regenerative programs might be turned on after injury in zebrafish brain and enable regenerative neurogenesis. Here we identify one such gene—the transcription factor gata3—which is expressed only after injury in different zebrafish organs. Gata3 is required for reactive proliferation of radial glia cells, subsequent regenerative neurogenesis, and migration of the newborn neurons. We found that these regeneration-specific roles of Gata3 are dependent on the injury because Gata3 overexpression in the unlesioned adult zebrafish brain is not sufficient to induce neurogenesis. Thus, gata3 acts as a specific injury-induced proregenerative factor that is essential for the regenerative capacity in vertebrates. [ABSTRACT FROM AUTHOR]