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Start Over Author "Cheng, Melodyanne Y." Topic transcription factors
2 results on '"Cheng, Melodyanne Y."'

1. Posttranscriptional Regulation of Gcr1 Expression and Activity Is Crucial for Metabolic Adjustment in Response to Glucose Availability.

Author

Hossain MA, Claggett JM, Edwards SR, Shi A, Pennebaker SL, Cheng MY, Hasty J, and Johnson TL

Subjects
Adaptation, Physiological, Exons, Gene Expression Regulation, Fungal, Genotype, Glucose deficiency, Introns, Mutation, Phenotype, Protein Isoforms, Protein Multimerization, RNA Splicing, Saccharomyces cerevisiae growth & development, Time Factors, Transcription, Genetic, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Glucose metabolism, Glycolysis genetics, RNA Processing, Post-Transcriptional, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Transcription Factors genetics, Transcription Factors metabolism
Abstract

The transcription factor Gcr1 controls expression of over 75% of the genes in actively growing yeast. Yet despite its widespread effects, regulation of Gcr1 itself remains poorly understood. Here, we show that posttranscriptional Gcr1 regulation is nutrient dependent. Moreover, GCR1 RNA contains a long, highly conserved intron, which allows the cell to generate multiple RNA and protein isoforms whose levels change upon glucose depletion. Intriguingly, an isoform generated by intron retention is exported from the nucleus, and its translation is initiated from a conserved, intronic translation start site. Expression of gene products from both the spliced and unspliced RNAs is essential, as cells expressing only one isoform cannot adjust their metabolic program in response to environmental changes. Finally, we show that the Gcr1 proteins form dimers, providing an elegant mechanism by which this one gene, through its regulation, can perform the repertoire of transcriptional activities necessary for fine-tuned environmental response., (Copyright © 2016 Elsevier Inc. All rights reserved.)

Published
2016
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2. Posttranscriptional Regulation of Gcr1 Expression and Activity Is Crucial for Metabolic Adjustment in Response to Glucose Availability

Author

Hossain, Munshi Azad, Claggett, Julia M, Edwards, Samantha R, Shi, Aishan, Pennebaker, Sara L, Cheng, Melodyanne Y, Hasty, Jeff, and Johnson, Tracy L

Subjects
RNA Processing, Saccharomyces cerevisiae Proteins, Time Factors, Genotype, Physiological, RNA Splicing, 1.1 Normal biological development and functioning, Post-Transcriptional, Saccharomyces cerevisiae, Medical and Health Sciences, Genetic, Underpinning research, Genetics, Protein Isoforms, Adaptation, Nutrition, Prevention, Exons, Biological Sciences, Introns, DNA-Binding Proteins, Glucose, Fungal, Phenotype, Gene Expression Regulation, Mutation, Generic health relevance, Protein Multimerization, Transcription, Glycolysis, Transcription Factors, Biotechnology, Developmental Biology
Abstract

The transcription factor Gcr1 controls expression of over 75% of the genes in actively growing yeast. Yet despite its widespread effects, regulation of Gcr1 itself remains poorly understood. Here, we show that posttranscriptional Gcr1 regulation is nutrient dependent. Moreover, GCR1 RNA contains a long, highly conserved intron, which allows the cell to generate multiple RNA and protein isoforms whose levels change upon glucose depletion. Intriguingly, an isoform generated by intron retention is exported from the nucleus, and its translation is initiated from a conserved, intronic translation start site. Expression of gene products from both the spliced and unspliced RNAs is essential, as cells expressing only one isoform cannot adjust their metabolic program in response to environmental changes. Finally, we show that the Gcr1 proteins form dimers, providing an elegant mechanism by which this one gene, through its regulation, can perform the repertoire of transcriptional activities necessary for fine-tuned environmental response.

Published
2016

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