1. Functional outcome of cell seeded tracheal scaffold after mechanical stress in vitro.
- Author
-
de Wit RJJ, Tiemessen D, Oosterwijk E, and Verhagen AFTM
- Subjects
- Animals, Swine, Chondrogenesis, Cells, Cultured, Bioreactors, Glycosaminoglycans metabolism, Tissue Scaffolds chemistry, Trachea cytology, Tissue Engineering methods, Stress, Mechanical
- Abstract
Tracheal tissue engineering is still facing major challenges: realization of efficient vascularization and mechanical properties comparable to native trachea need to be achieved. In this study, we present a strategy for the manufacturing of a construct for tracheal tissue engineering by conditioning through cell seeding followed by mechanical stimulation in vitro. Scaffolds derived from porcine trachea decellularized with supercritical carbon dioxide were seeded with stem cells of different tissue sources and cultured in a bioreactor for 21 days under mechanical stimulation. Enhanced chondrogenic development was demonstrated, with improved sulphated glycosaminoglycan secretion and cellular alignment which resulted in mechanical properties resembling native trachea. This method may provide a useful addition to tracheal tissue engineering strategies aimed at optimizing cartilage formation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2025
- Full Text
- View/download PDF