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Start Over Author "Du, Shuhua" Topic trabecular meshwork
3 results on '"Du, Shuhua"'

1. [Effect of pressure on proliferation and apoptosis of bovine trabecular meshwork].

Author

Xue W, Du S, and Li Y

Subjects
Animals, Cattle, Cell Division physiology, Cells, Cultured, In Situ Nick-End Labeling, Pressure, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-bcl-2 genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Time Factors, Trabecular Meshwork cytology, bcl-2-Associated X Protein, Apoptosis physiology, Trabecular Meshwork metabolism
Abstract

Objective: To investigate the effect of pressure on the expression of cell cycle and apoptosis related gene bcl-2 and bax mRNA by bovine trabecular meshwork cells., Method: Twenty mm Hg, 40 mm Hg, 60 mm Hg and 80 mm Hg(1 mm Hg = 0.133 kPa) pressure were given to the cultured trabecular meshwork cells respectively in the treatment groups. No pressure was given in the control groups. Each pressure sustained for 24 and 48 hours. Proliferation index, apoptosis index, bcl-2 and bax mRNA expression of trabecular meshwork cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling, flow cytometry and in situ hybridization., Results: In 24 hour groups, there were no significant differences between the apoptosis index of control group and pressure /= 60 mm Hg. In 48 hour groups, apoptosis index, proliferative index and bax mRNA expression had significant differences from that of the control groups when pressure was >/= 40 mm Hg., Conclusion: Pressure >/= 40 mm Hg sustaining for a period of time can suppress trabecular meshwork cell proliferation and induce apoptosis through influencing bcl-2 family members.

Published
2002

2. Effects of nitric oxide on proliferation and apoptosis of cultured bovine trabecular meshwork cells.

Author

Xue W, Du S, Li Y, Yang Y, and Sun J

Subjects
Animals, Cattle, Cell Division drug effects, Cells, Cultured, Culture Media, Cyclin D1 biosynthesis, Cyclin D1 genetics, NG-Nitroarginine Methyl Ester pharmacology, Proto-Oncogene Proteins biosynthesis, Proto-Oncogene Proteins genetics, RNA, Messenger biosynthesis, RNA, Messenger genetics, bcl-2-Associated X Protein, Apoptosis drug effects, Nitric Oxide pharmacology, Proto-Oncogene Proteins c-bcl-2, Trabecular Meshwork cytology
Abstract

The effects of different doses of nitric oxide (NO) on the proliferation and apoptosis of the cultured bovine trabecular meshwork (TM) cells were studied. L-arginine and NG-nitro-L-arginine methyl (L-NAME) were incubated with TM cells for 48 h. In the control group, no medicine was given. In the experimental groups, concentrations of L-arginine and L-NAME were 1 x 10(-7) mol/L, 1 x 10(-6) mol/L, 1 x 10(-5) mol/L, 1 x 10(-4) mol/L, 1 x 10(-3) mol/L and 1 x 10(-2) mol/L, respectively. NO2- in supernate, the proliferation and apoptosis of TM cells and mRNA expression of bcl-2 and bax were measured by Griess reagent, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), MTT assay and in situ hybridization, respectively. The results showed that L-arginine with concentration > or = 1 x 10(-4) mol/L could induce apoptosis of the TM cells and inhibit the proliferation of TM cells through increasing the NO levels, down-regulating bcl-2 mRNA expression and up-regulating bax mRNA expression; L-NAME with concentration > or = 1 x 10(-5) mol/L could induce the proliferation of the TM cells through suppressing the production of NO. It was concluded that NO in high level could induce apoptosis of the TM cells and suppress the proliferation of the TM cells.

Published
2002
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3. Effects of nitric oxide on proliferation and apoptosis of cultured bovine trabecular meshwork cells

Author

Yang Yejing, Du Shuhua, Sun Jinghua, Xue Wei, and Li Yong

Subjects
Biomedical Engineering, Apoptosis, In situ hybridization, Biology, Nitric Oxide, Biochemistry, Nitric oxide, Biomaterials, chemistry.chemical_compound, Griess test, Trabecular Meshwork, Proto-Oncogene Proteins, Genetics, medicine, Animals, MTT assay, Cyclin D1, RNA, Messenger, Cells, Cultured, Earth-Surface Processes, bcl-2-Associated X Protein, TUNEL assay, Molecular biology, Culture Media, medicine.anatomical_structure, NG-Nitroarginine Methyl Ester, Terminal deoxynucleotidyl transferase, chemistry, Proto-Oncogene Proteins c-bcl-2, Cattle, Trabecular meshwork, Cell Division
Abstract

The effects of different doses of nitric oxide (NO) on the proliferation and apoptosis of the cultured bovine trabecular meshwork (TM) cells were studied. L-arginine and NG-nitro-L-arginine methyl (L-NAME) were incubated with TM cells for 48h. In the control group, no medicine was given. In the experimental groups, concentrations of L-arginine and L-NAME were 1×10−7 mol/L, 1×10−6 mol/L, 1×10−5 mol/L, 1×10−4 mol/L, 1×10−3 mol/L and 1×10−2 mol/L, respectively. NO 2 − in supernate, the proliferation and apoptosis of TM cells and mRNA expression of bcl-2 and bax were measured by Griess reagent, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), MTT assay and in situ hybridization, respectively. The results showed that L-arginine with concentration ≥1×10−4 mol/L could induce apoptosis of the TM cells and inhibit the proliferation of TM cells through increasing the NO levels, down-regulating bcl-2 mRNA expression and up-regulating bax mRNA expression; L-NAME with concentration ≥1×10−5 mol/L could induce the proliferation of the TM cells through suppressing the production of NO. It was concluded that NO in high level could induce apoptosis of the TM cells and suppress the proliferation of the TM cells.

Published
2003

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