1. Reciprocal influence of hMSCs/HaCaT cultivated on electrospun scaffolds.
- Author
-
Bhowmick S, Rother S, Zimmermann H, Lee PS, Moeller S, Schnabelrauch M, Koul V, and Scharnweber D
- Subjects
- Cell Differentiation, Cells, Cultured, Coculture Techniques instrumentation, Coculture Techniques methods, Electroplating methods, Gene Expression Regulation, Humans, Materials Testing, Nanofibers chemistry, Transcription Factors genetics, Transcription Factors metabolism, Tumor Suppressor Proteins genetics, Tumor Suppressor Proteins metabolism, Cell Communication physiology, Keratinocytes cytology, Keratinocytes physiology, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells physiology, Tissue Scaffolds chemistry
- Abstract
Here, we investigated the synergistic effect of electrospun nanofibrous scaffolds made of gelatin /sulfated hyaluronan (sHA) or native hyaluronan (HA)/chondroitin sulfate (CS) and, keratinocytes (HaCaT)-human mesenchymal stem cells (hMSCs) contact co-culture on epithelial differentiation of hMSCs. The hMSCs were co-cultured in contact with HaCaT cells for 5 days on electrospun scaffold. Results show that electrospun scaffolds containing sulfated glycosaminoglycans (GAGs) stimulate epithelial differentiation in terms of various protein expression markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of several dermal proteins (keratin 14, ΔNp63α). Electrospun scaffold independent of GAGs alone did not affect the epithelial differentiation of hMSCs but combination of keratinocyte-hMSC contact co-culture and electrospun scaffold promotes the epithelial differentiation of hMSCs.
- Published
- 2017
- Full Text
- View/download PDF