1. FLight Biofabrication Supports Maturation of Articular Cartilage with Anisotropic Properties.
- Author
-
Puiggalí-Jou A, Rizzo R, Bonato A, Fisch P, Ponta S, Weber DM, and Zenobi-Wong M
- Subjects
- Animals, Anisotropy, Chondrocytes cytology, Chondrocytes metabolism, Collagen chemistry, Collagen metabolism, Cattle, Porosity, Cartilage, Articular metabolism, Cartilage, Articular physiology, Tissue Engineering methods, Tissue Scaffolds chemistry, Extracellular Matrix metabolism, Extracellular Matrix chemistry
- Abstract
Tissue engineering approaches that recapitulate cartilage biomechanical properties are emerging as promising methods to restore the function of injured or degenerated tissue. However, despite significant progress in this research area, the generation of engineered cartilage constructs akin to native counterparts still represents an unmet challenge. In particular, the inability to accurately reproduce cartilage zonal architecture with different collagen fibril orientations is a significant limitation. The arrangement of the extracellular matrix (ECM) plays a fundamental role in determining the mechanical and biological functions of the tissue. In this study, it is shown that a novel light-based approach, Filamented Light (FLight) biofabrication, can be used to generate highly porous, 3D cell-instructive anisotropic constructs that lead to directional collagen deposition. Using a photoclick-based photoresin optimized for cartilage tissue engineering, a significantly improved maturation of the cartilaginous tissues with zonal architecture and remarkable native-like mechanical properties is demonstrated., (© 2023 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF