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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Journal of Oral & Maxillofacial Surgery is the property of Shanghai Jiao Tong University, College of Stomatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: All-natural cardiac decellularized scaffold material has macroscopic and microstructure, matrix components and vascular network distribution similar to the receptor, which is an ideal material for heart tissue engineering. OBJECTIVE: To summarize the preparation methods, composition characteristics, biological characteristics and the latest research progress of cardiac decellularized matrix scaffold. METHODS: Relevant articles published from January 2008 to April 2017 were searched in PubMed and Wanfang databases using the keywords of “heart, cardiac muscle, myocardial tissue, decellularized matrix” in English and Chinese, respectively. Finally, 50 representative articles (44 in English and 6 in Chinese) were included with the exception of the articles that were associated with cardiac valves. RESULTS AND CONCLUSION: Currently, the methods of preparing cardiac decellular matrix scaffolds include physical processing, chemical method and biological treatment (enzymatic method). Cardiac extracellular matrix scaffolds mainly contain I, III and IV collagen, glycosaminoglycans, fibronectin, laminin and a small amount of growth factors. At present, the application of the decellular matrix in myocardial tissue engineering includes three directions: the "band-aid" myocardial tissue engineering study based on decellular matrix lamella; the study of the myocardial tissue engineering on injectable myocardial tissue engineering based on the decellular matrix; and the study of decellularized-recellularized artificial cardiac reengineering based on the cardiac all-organ. Although some successful experience has been achieved in the stents, their use in the cardiac replantation still has many problems to be solved. [ABSTRACT FROM AUTHOR]

BACKGROUND: In recent years, it has shown that there exist some endogenous cardiac stem cells in the heart. What has been confirmed is that this kind of cells can differentiate into cardiomyocytes to repair the damaged myocardium and improve the cardiac function. OBJECTIVE: To review the current methods of promoting the differentiation of cardiac stem cells into cardiomyocytes. METHODS: PubMed database was searched by computer for articles addressing the differentiation of cardiac stem cells in the last 10 years, using the keywords of "cardiac stem cells, cardiac progenitor cells, differentiation". Finally, 64 English articles were included in result analysis. RESULTS AND CONCLUSION: Recent studies have shown that the differentiation efficiency of cardiac stem cells can be promoted in vivo by introducing cytokines, micro-RNA, and some physicochemical methods, which consequently enhances the therapeutic efficacy of cardiac stem cell transplantation for myocardial infarction. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: An ideal animal model is important for studying gouty arthritis. However, a lack of perfect animal model of gouty arthritis delays the progress in searching for a novel drug and treatment method. OBJECTIVE: To evaluate the effectiveness of the modified rabbit model of gouty knee arthritis. METHODS: New Zealand white rabbits were selected and randomly divided into four groups. Except blank control group received no intervention; the rabbit greater omentum was removed, which was wrapped with nothing (sham operation group), implanted with 0.1 g/L monosodium urate crystal suspension (conventional group), or 100 mg/kg monosodium urate crystal (modified group) into the suprapatellar cyst of the rabbit right knee. The swelling degree, motor function, and inflammatory response of the knee joint were observed at 1, 2 and 3 days, 1, 2, 3, and 4 weeks after modeling to evaluate the advantages and disadvantages of the modified model. RESULTS AND CONCLUSION: The movement function in the modified group was significantly decreased within < weeks after modeling (P < 0.05). The joint swelling index in the conventional and modified groups peaked on day 1 or 2, and then began to decrease. The descent velocity in the modified group was slower than that in the conventional group, and the swelling index showed significant difference between modified and blank control groups at 4 weeks after modeling (P < 0.05). The conventional and modified groups presented with overt inflammatory response, synovial hyperplasia, congestion and edema; abundant synovial fluid appeared in the articular cavity; periphery tissue became congestion and edema; the cartilage revealed no obvious damage. The levels of interleukin-6 and -1β in serum and synovial fluid in the conventional and modified groups peaked on days 1 and 2, followed by a decrease, but the modified group exhibited a slower descent velocity compared with the modified group. All above levels in the modified group were significantly higher than those in the other three groups (P < 0.05). These results suggest that the rabbit model of gouty knee arthritis is successful established by the intra-articular implantation with the autogenous greater omentum-wrapped monosodium urate crystal, which can last more than 4 weeks, longer than the conventional model. [ABSTRACT FROM AUTHOR]

BACKGROUND: Spinal cord injury is a damage to the spinal cord with a high morbidity that can be divided into primary and secondary injury. Secondary injury does more harm to the body than primary injury, which can be regulated and improved through proper interventions. In addition to the drugs, surgical decompression and other traditional treatments, hypothermia is an important physical intervention that has been shown to regulate secondary injury following spinal cord injury, and hold neuroprotective effect. OBJECTIVE: To introduce different hypothermia treatments for spinal cord injury and the effect on the disordered environment after spinal cord injury, as well as summarize the latest progression. METHODS: A computer-based search of CNKI and PubMed databases was conducted for the articles addressing the application of hypothermia in spinal cord injury published from January 2001 to June 2016, using the keywords of “therapeutic hypothermia or low temperature, spinal cord injury” in Chinese and English, respectively. RESULTS AND CONCLUSION: Hypothermia is divided into systematic and local hypothermia, and the former is simple and convenient, but it may lead to complications. Local hypothermia can quickly reach the target temperature, to make deep hypothermia at injury site and stablize the core temperature in the body, but it is invasive, and the necessary time of locating and maintaining effective temperature is a challenge. In general, hypothermia can improve the disordered microenvironment after spinal cord injury, reduce inflammatory infiltration, regulate the expression of relative genes and proteins, and promote the proliferation and differentiation of endogenous nerve cells. There are basic and clinical studies on hypothermia neuroprotection against spinal cord injury from various aspects; thereafter, hypothermia is a promising treatment strategy for spinal cord injury. [ABSTRACT FROM AUTHOR]