1. Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization
- Author
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Elena N. Golubeva, Ekaterina A. Grebenik, Elvira R. Gafarova, A. V. Kurkov, Anna B. Soloviova, Byron S. Kapomba, Anastasia Frolova, Ilya A. Bazhanov, Alexey E. Lazhko, Peter S. Timashev, I.A. Novikov, Nastasia V. Kosheleva, Anatoly B. Shekhter, and Anastasia S. Kuryanova
- Subjects
Aortic valve ,Donor tissue ,Aortic root ,0206 medical engineering ,Pharmaceutical Science ,02 engineering and technology ,Time cost ,Article ,Analytical Chemistry ,supercritical CO2 ,lcsh:QD241-441 ,Tissue engineering ,lcsh:Organic chemistry ,Elastic Modulus ,Tensile Strength ,biomedical engineering ,Drug Discovery ,Ultimate tensile strength ,medicine ,Animals ,Humans ,Physical and Theoretical Chemistry ,Cells, Cultured ,Sheep ,Decellularization ,Tissue Engineering ,Chemistry ,Organic Chemistry ,Mesenchymal Stem Cells ,Carbon Dioxide ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,aortic valve ,Cellular Structures ,Supercritical fluid ,Extracellular Matrix ,medicine.anatomical_structure ,Chemistry (miscellaneous) ,Molecular Medicine ,decellularization ,0210 nano-technology ,Biomedical engineering ,biomaterials - Abstract
One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from a donor tissue, reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2)-assisted processing has been proposed to improve the outcome of decellularization, reduce contamination and time costs. The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, the decellularization of heterogeneous 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, the type of co-solvent, values of pressure and temperature inside the scCO2 reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the effectiveness of decellularization approaches based on various combinations of these parameters. The protocols were based on the combinations of treatments in alkaline, ethanol or detergent solutions with scCO2-assisted processing at different modes. Histological analysis demonstrated favorable effects of the preconditioning in a detergent solution. Following processing in scCO2 medium provided a high decellularization degree, reduced cytotoxicity, and increased ultimate tensile strength and Young&rsquo, s modulus of the aortic valve leaflets, while the integrity of the extracellular matrix was preserved.
- Published
- 2020
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