9 results on '"Hutcheson HJ"'
Search Results
2. Experimental transmission of West Nile virus (Flaviviridae: Flavivirus) by Carios capensis ticks from North America.
- Author
-
Hutcheson HJ, Gorham CH, Machain-Williams C, Loroño-Pino MA, James AM, Marlenee NL, Winn B, Beaty BJ, and Blair CD
- Subjects
- Animals, Bird Diseases parasitology, RNA, Viral analysis, Reverse Transcriptase Polymerase Chain Reaction veterinary, Viremia veterinary, West Nile Fever parasitology, Arachnid Vectors virology, Bird Diseases transmission, Ducks parasitology, Ticks virology, West Nile Fever transmission, West Nile virus isolation & purification
- Abstract
Seabird soft ticks, Carios capensis (Ixodida: Argasidae), originally collected from coastal Georgia, USA, were allowed to ingest a blood meal from pekin ducklings (Anas domesticus) infected with WNV. After 35 days of extrinsic incubation, the ticks transmitted virus to naive ducklings. WNV was detected via plaque assay and RTPCR in ticks and in tissues and serum of ducklings 7 days post infestation.
- Published
- 2005
- Full Text
- View/download PDF
3. Ticks and antibodies to Borrelia burgdorferi from mammals at Cape Hatteras, NC and Assateague Island, MD and VA.
- Author
-
Oliver JH Jr, Magnarelli LA, Hutcheson HJ, and Anderson JF
- Subjects
- Animals, Cats, Female, Horses, Host-Parasite Interactions, Lyme Disease immunology, Mammals, Maryland, Mice, North Carolina, Rats, Virginia, Antibodies, Bacterial analysis, Borrelia burgdorferi Group immunology, Lyme Disease veterinary, Ticks microbiology
- Abstract
Results of a survey for ixodid ticks and/or serum antibodies to Borrelia burgdorferi from 14 species of small to large mammals from eastern coastal areas of the United States are presented. Most samples were obtained from July 1987 through June 1989 (excluding December-March) at 3 locales: Assateague Is. National Seashore, Worcester Co., MD., and Accomack Co., VA. (approximately 38 degrees 05' N 75 degrees 10' W), and Cape Hatteras National Seashore, Dare Co., NC (approximately 35 degrees 30' N 76 degrees 35' W). Hosts sampled included opossums (Didelphis virginiana), least shrews (Cryptotis parva), gray foxes (Urocyon cinereoargenteus), red foxes (Vulpes vulpes), raccoons (Procyon lotor), feral cats (Felis sylvestris), feral horses (Equus caballus), sika deer (Cervus nippon), rice rats (Oryzomys palustris), white-footed mice (Peromyscus leucopus), meadow voles (Microtus pennsylvanicus), house mice (Mus musculus), norway rats (Rattus norvegicus) and jumping mice (Zapus hudsonius). An indirect fluorescent antibody test was used for testing sera from opossums, raccoons, and feral cats; enzyme-linked immunosorbent assays were used for sera from foxes, horses, deer, and house and white-footed mice. Antibodies to B. burgdorferi were found in all species tested from each locale. Seasonal data reinforce the contention that P. leucopus is a suitable sentinel species for B. burgdorferi. Ticks on hosts included Ixodes scapularis Say, I. texanus Banks, Dermacentor variabilis (Say), D. albipictus (Packard), and Amblyomma americanum (L.). Males comprised approximately 0-22 and 60-81% of Ixodes sp. and Amblyomma-Dermacentor adults collected from hosts, respectively. All stages of A. americanum, adult D. variabilis, and larval I. scapularis were collected from vegetation. The highest seropositivity rate (67%) was recorded for 45 P. leucopus at Assateague during July, approximately 1 mo. after peak nymphal I. scapularis intensity. Borrelia burgdorferi was isolated from 6 nymphal and 12 female I. scapularis collected from P. leucopus and C. nippon, respectively, on Assateague.
- Published
- 1999
- Full Text
- View/download PDF
4. Multivariate morphometric discrimination of nymphal and adult forms of the blacklegged tick (Acari: Ixodidae), a principal vector of the agent of Lyme disease in eastern North America.
- Author
-
Hutcheson HJ, Oliver JH Jr, Houck MA, and Strauss RE
- Subjects
- Animals, Female, Lyme Disease parasitology, Male, New England, Nymph, Phenotype, Phylogeny, Arthropod Vectors anatomy & histology, Genetic Variation, Ticks anatomy & histology
- Abstract
A morphological study of postlarval stages of the blacklegged tick, Ixodes scapularis Say, was conducted to examine congruence between northern (formerly I. dammini Spielman, Clifford, Piesman & Corwin) and southern morphotypes. The character set totaled 17 for females, 25 for males, and 28 for nymphs. Populations from 6 geographic areas, F1 progeny from reciprocal crosses between I. scapularis from Massachusetts and Georgia, and I. pacificus Cooley & Kohls from California were measured. Characters, except cornua length in nymphs, were positively correlated with PC1, indicating it was a general-size eigenvector. Characters used previously by others to distinguish northern and southern forms had a highly positive allometric relationship to general size. In canonical variate analysis (CVA) of nymphs, canonical score 1 (CAN1) discriminated I. pacificus from all other groups, canonical score 2 (CAN2) discriminated the remaining groups in a pattern that correlated with latitude, and canonical score 3 (CAN3) separated the western (Minnesota and Missouri) from the eastern groups. Size-free CVA indicated that north-south discrimination was size dependent, but that variation between Missouri and all other groups was not size related. Mahalanobis distances between groups within stages were significant with the exception of the 2 groups of female progeny of reciprocal crosses (Massachusetts x Georgia). Analysis of variance and Student-Newman-Keuls tests revealed that each geographic population differed from all other groups in at least 1 nymphal character. Nymphs from northern areas (Minnesota, Massachusetts, Maryland) differed from those from southern areas (Missouri, North Carolina, Georgia) in characters associated with the basis capitulum (longer intercornua and interauricular distances, basis capituli, and hypostome internal files, larger auriculae, but shorter cornua). Western populations (Minnesota, Missouri) differed from eastern populations in idiosomal characters (broader scutum, larger coxae III and IV). Frequency polygons of characters with the greatest differences indicated that data are continuous and geographic variation is overlapping. Thus, the data support the previous contention of conspecificity of I. scapularis and I. dammini. I. scapularis appears to be a polytypic species with a widespread geographic distribution exhibiting north-south and east-west morphological clines in eastern North America.
- Published
- 1995
- Full Text
- View/download PDF
5. Conspecificity of the ticks Ixodes scapularis and I. dammini (Acari: Ixodidae).
- Author
-
Oliver JH Jr, Owsley MR, Hutcheson HJ, James AM, Chen C, Irby WS, Dotson EM, and McLain DK
- Subjects
- Animals, Crosses, Genetic, Female, Hybridization, Genetic, Isoenzymes analysis, Lyme Disease transmission, Male, Arachnid Vectors classification, Ticks classification
- Abstract
Reciprocal crosses between Ixodes dammini Spielman, Clifford, Piesman & Corwin from Massachusetts and Ixodes scapularis Say from Georgia produced offspring through the F3 generation when the experiment was discontinued. Reciprocal I. dammini x Ixodes pacificus Cooley & Kohls (California) and I. scapularis x I. pacificus crosses produced F1 progeny; however, all progeny were sterile. Assortative mating experiments between I. dammini and I. scapularis indicated that males and females of both species mated with the opposite sex of heterospecific or conspecific ticks when there was a choice. Conventional discriminant analysis of morphometric measurements of ticks from Georgia, North Carolina, Maryland, Massachusetts, and two populations of F1 hybrids indicated that there were recognizable differences. However, size-free (sheared) discriminant analysis indicated that these differences were largely size-dependent, with much overlap of the four eastern and two hybrid populations but no overlap with I. pacificus from California. Analysis of chromosomes (morphology and C band) indicated no differences between the Georgia and Massachusetts populations but showed a difference between them and the California population of I. pacificus. Analysis of isozymes showed that the genetic identity value for the Georgia and Massachusetts populations was within the normal range for conspecific populations, whereas the California population indicated congeneric but not conspecific relatedness to the Georgia and Massachusetts populations. Life cycle data collected under similar laboratory conditions showed no differences in length of feeding and molting periods among Georgia, Massachusetts, and California populations. These data and results of the work of other authors on tick host preferences and vector competence indicate that I. dammini is not a valid species separate from I. scapularis. Because the name Ixodes scapularis Say, 1821, has priority over the name Ixodes dammini Spielman, Clifford, Piesman & Corwin, 1979, I. dammini is relegated to a junior subjective synonym of I. scapularis (based on Article 23 of the International Code of Zoological Nomenclature).
- Published
- 1993
- Full Text
- View/download PDF
6. Ornithodoros (Alectorobius) capensis Neumann (Acari: Ixodoidea: Argasidae), a parasite of seabirds, established along the southeastern seacoast of the United States.
- Author
-
Keirans JE, Hutcheson HJ, and Oliver JH Jr
- Subjects
- Animals, Atlantic Ocean, Birds, Southeastern United States, Tick Infestations parasitology, Bird Diseases parasitology, Tick Infestations veterinary, Ticks isolation & purification
- Abstract
Ornithodoros (Alectorobius) capensis Neumann, an ectoparasite of seabirds found circumglobally in the tropics and subtropics, has become established along the southeastern seacoast of the United States. The tick has been found feeding primarily on brown pelicans, Pelecanus occidentalis, but also has been found on the laughing gull, Larus atricilla, and the American oystercatcher, Haematopus palliatus. We report here the presence of O. (A.) capensis from New Hanover and Brunswick counties (near the mouth of the Cape Fear River) in North Carolina, to the Charleston Harbor area of South Carolina and thence south to Cumberland Island (a barrier island) in Camden County, Georgia, just north of the Florida state line.
- Published
- 1992
- Full Text
- View/download PDF
7. Chromosomes of the tropical horse tick, Dermacentor nitens (Acari: Ixodidae), with notes on the life cycle.
- Author
-
Oliver JH Jr, Owsley MR, and Hutcheson HJ
- Subjects
- Animals, Dermacentor growth & development, Dermacentor physiology, Feeding Behavior, Female, Male, Oviposition, Sex Chromosomes, Sex Determination Analysis, Chromosomes, Dermacentor genetics, Ticks genetics
- Published
- 1987
8. Multivariate morphometric discrimination of nymphal and adult forms of the blacklegged tick (Acari: Ixodidae), a principal vector of the agent of Lyme disease in eastern North America
- Author
-
Oliver Jh, Hutcheson Hj, Richard E. Strauss, and Houck Ma
- Subjects
Male ,Nymph ,Tick ,Ticks ,New England ,Animals ,Acari ,Phylogeny ,Lyme Disease ,General Veterinary ,biology ,Ecology ,Arthropod Vectors ,Genetic Variation ,Parasitiformes ,biology.organism_classification ,Infectious Diseases ,Phenotype ,Ixodes scapularis ,Insect Science ,Ixodes pacificus ,Parasitology ,Female ,Allometry ,Ixodidae - Abstract
A morphological study of postlarval stages of the blacklegged tick, Ixodes scapularis Say, was conducted to examine congruence between northern (formerly I. dammini Spielman, Clifford, Piesman & Corwin) and southern morphotypes. The character set totaled 17 for females, 25 for males, and 28 for nymphs. Populations from 6 geographic areas, F1 progeny from reciprocal crosses between I. scapularis from Massachusetts and Georgia, and I. pacificus Cooley & Kohls from California were measured. Characters, except cornua length in nymphs, were positively correlated with PC1, indicating it was a general-size eigenvector. Characters used previously by others to distinguish northern and southern forms had a highly positive allometric relationship to general size. In canonical variate analysis (CVA) of nymphs, canonical score 1 (CAN1) discriminated I. pacificus from all other groups, canonical score 2 (CAN2) discriminated the remaining groups in a pattern that correlated with latitude, and canonical score 3 (CAN3) separated the western (Minnesota and Missouri) from the eastern groups. Size-free CVA indicated that north-south discrimination was size dependent, but that variation between Missouri and all other groups was not size related. Mahalanobis distances between groups within stages were significant with the exception of the 2 groups of female progeny of reciprocal crosses (Massachusetts x Georgia). Analysis of variance and Student-Newman-Keuls tests revealed that each geographic population differed from all other groups in at least 1 nymphal character. Nymphs from northern areas (Minnesota, Massachusetts, Maryland) differed from those from southern areas (Missouri, North Carolina, Georgia) in characters associated with the basis capitulum (longer intercornua and interauricular distances, basis capituli, and hypostome internal files, larger auriculae, but shorter cornua). Western populations (Minnesota, Missouri) differed from eastern populations in idiosomal characters (broader scutum, larger coxae III and IV). Frequency polygons of characters with the greatest differences indicated that data are continuous and geographic variation is overlapping. Thus, the data support the previous contention of conspecificity of I. scapularis and I. dammini. I. scapularis appears to be a polytypic species with a widespread geographic distribution exhibiting north-south and east-west morphological clines in eastern North America.
- Published
- 1995
9. Conspecificity of the ticks Ixodes scapularis and I. dammini (Acari: Ixodidae)
- Author
-
Denson Kelly McLain, W. S. Irby, M. R. Owsley, J. H. Oliver, Hutcheson Hj, Angela M. James, Chunsheng Chen, and Ellen M. Dotson
- Subjects
Male ,Population ,Tick ,Ticks ,Animals ,Acari ,education ,Crosses, Genetic ,education.field_of_study ,Lyme Disease ,General Veterinary ,biology ,Ecology ,Assortative mating ,Parasitiformes ,biology.organism_classification ,Isoenzymes ,Infectious Diseases ,Ixodes scapularis ,Insect Science ,Ixodes pacificus ,Hybridization, Genetic ,Parasitology ,Arachnid Vectors ,Female ,Ixodidae - Abstract
Reciprocal crosses between Ixodes dammini Spielman, Clifford, Piesman & Corwin from Massachusetts and Ixodes scapularis Say from Georgia produced offspring through the F3 generation when the experiment was discontinued. Reciprocal I. dammini × Ixodes pacificus Cooley & Kohls (California) and I. scapularis × I. pacificus crosses produced F1 progeny; however, all progeny were sterile. Assortative mating experiments between I. dammini and I. scapularis indicated that males and females of both species mated with the opposite sex of heterospecific or conspecific ticks when there was a choice. Conventional discriminant analysis of morphometric measurements of ticks from Georgia, North Carolina, Maryland, Massachusetts, and two populations of F1 hybrids indicated that there were recognizable differences. However, size-free (sheared) discriminant analysis indicated that these differences were largely size-dependent, with much overlap of the four eastern and two hybrid populations but no overlap with I. pacificus from California. Analysis of chromosomes (morphology and C band) indicated no differences between the Georgia and Massachusetts populations but showed a difference between them and the California population of I. pacificus. Analysis of isozymes showed that the genetic identity value for the Georgia and Massachusetts populations was within the normal range for conspecific populations, whereas the California population indicated congeneric but not conspecific relatedness to the Georgia and Massachusetts populations. Life cycle data collected under similar laboratory conditions showed no differences in length of feeding and molting periods among Georgia, Massachusetts, and California populations. These data and results of the work of other authors on tick host preferences and vector competence indicate that I. dammini is not a valid species separate from I. scapularis. Because the name Ixodes scapularis Say, 1821, has priority over the name Ixodes dammini Spielman, Clifford, Piesman & Corwin, 1979, I. dammini is relegated to a junior subjective synonym of I. scapularis (based on Article 23 of the International Code of Zoological Nomenclature).
- Published
- 1993
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.