1. An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi.
- Author
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Wasser MN, Koppert PW, Arndt JW, Emeis JJ, Feitsma RI, Pauwels EK, and Nieuwenhuizen W
- Subjects
- Abdomen blood supply, Animals, Binding Sites, Antibody, Fibrin immunology, Fibrinogen immunology, Immunoenzyme Techniques, Jugular Veins, Male, Mice, Mice, Inbred BALB C, Rabbits, Radionuclide Imaging, Rats, Technetium, Vena Cava, Inferior, Antibodies, Monoclonal, Thrombosis diagnostic imaging
- Abstract
Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 kappa-type and appear to be directed against a conformation-dependent epitope in the D-domain of fibrin. Experiments with 99mTc-labeled Y22 in vitro show that Y22 binds rapidly to forming clots. 99mTc-Y22 also binds to preformed plasma clots in a plasma milieu, even in the presence of high concentrations of heparin. Clot localization experiments in rabbits and rats confirm the high fibrin specificity and the potential of 99mTc-Y22 for thrombus imaging in vivo.
- Published
- 1989