1. No interplay between the pathways mediating coagulation and inflammation in tissue factor-induced disseminated intravascular coagulation in rats.
- Author
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Ontachi Y, Asakura H, Takahashi Y, Hayashi T, Arahata M, Kadohira Y, Maekawa M, Omote M, Yoshida T, Yamazaki M, Morishita E, Miyamoto K, and Nakao S
- Subjects
- Animals, Antifibrinolytic Agents pharmacology, Cytokines drug effects, Disseminated Intravascular Coagulation chemically induced, Interleukin-10 metabolism, Interleukin-6 metabolism, Lipopolysaccharides pharmacology, Male, Prospective Studies, Rats, Rats, Wistar, Tranexamic Acid pharmacology, Tumor Necrosis Factor-alpha metabolism, Cytokines metabolism, Disseminated Intravascular Coagulation physiopathology, Hemostatics pharmacology, Receptor Cross-Talk drug effects, Signal Transduction drug effects, Thromboplastin pharmacology
- Abstract
Objective: Previous reports have suggested an interplay between the pathways mediating coagulation and inflammation in endotoxemia and sepsis. The present study was designed to examine whether cross-signaling between the pathways mediating coagulation and inflammation occurs, as suggested by the pattern of cytokine production observed following tissue-factor (TF)-induced disseminated intravascular coagulation (DIC)., Design: Prospective, comparative, experimental study., Setting: Laboratory at a university hospital., Subjects: Male Wistar rats, aged 6-7 wks, and weighing 160-170 g., Interventions: Male Wistar rats were administered TF (3.75 units/kg every 4 hrs), TF, and tranexamic acid (TA; 50 mg/kg every 4.5 hrs) or lipopolysaccharide (30 mg/kg every 4 hrs) via the tail vein, and blood was sampled at 0, 4, 8 and 12 hrs., Measurements and Main Results: Subsequent alterations in thrombin-antithrombin complex and fibrinogen levels, as well as platelet counts, indicated that the severity of both types of experimental DIC (TF-induced and lipopolysaccharide-induced) was similar with respect to hemostatic activation and development of consumption coagulopathy. In lipopolysaccharide-induced DIC, a sharp increase in plasma tumor necrosis factor levels was observed at 4 hrs, after which a sharp decline was noted. Plasma levels of interleukin-6 were markedly increased at 4 hrs, after which a sustained elevation was observed for the duration of the experimental period (tumor necrosis factor, 1270 +/- 280, 180 +/- 40, and 120 +/- 30 pg/mL at 4, 8 and 12 hrs, respectively; interleukin-6, 5810 +/- 1320, 4850 +/- 730, and 5230 +/- 1280 pg/mL at 4, 8 and 12 hrs, respectively). On the other hand, tumor necrosis factor and interleukin-6 were not detected following TF-induced DIC (0 +/- 0 at 4, 8, and 12 hrs for both tumor necrosis factor and interleukin-6). In the TF+TA group, significant increases in tumor necrosis factor and interleukin-6 were observed, compared with the TF group., Conclusions: There is no overt interplay between the pathways mediating coagulation and inflammation in TF-induced DIC, as observed in lipopolysaccharide-induced DIC.
- Published
- 2006
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