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This study aims to investigate the engine performance of spark-ignition engines with port fuel injection using E50 fuel, which contains 50% gasoline or pertalite (in Indonesia) and 50% hydrate bioethanol, at airfuel ratio variations of 10:1, 12:1, and 14:1. The experiments were conducted using a 1-cylinder, 662 cc engine research test with a constant load of 3 kg and engine speed variations of 1500 and 1800 RPM, as well as a compression ratio of 10:1 and standard ignition timing. The engine ran with E50 fuel, and the experimental results were compared with those of E0 for an air-fuel ratio of 14:1. According to the data, the fuel energy of E50 in an AFR of 14:1 is 0.11 kW higher than that of E0, and it increases by 21.6% when the engine speed increases from 1500 to 1800 RPM. The results also indicate that the efficiency of all performance indicators, such as indicative thermal efficiency, brake thermal efficiency, and mechanical efficiency, is maximized when the engine is operated at an AFR of 10:1 for E50 fuel. Additionally, the volumetric efficiency of E50 reaches its maximum when the fuel is burned at an AFR of 14:1, and it increases as the engine speed increases. However, it should be noted that the brake power decreases due to the frictional power of the fuel increase. [ABSTRACT FROM AUTHOR]