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Bacterial cellulose is used in different areas. One of these areas is using this material as bioadsorbent for decolorization of different dyes. In the study, Gluconacetobacter xylinus was used for obtaining bacterial cellulose (BC). The wet and dry forms of the BC were utilized as bioadsorbent for removal of the Reactive Blue 171 (C.I Chrocion Blue HERD) (200 mg/L), Remazol Brilliant Blue R (150 mg/L) (C. I Reactive Blue 19), and Chrocion Green H-E4BD (100 mg/L) dyes. The highest decolorization rates were obtained with wet BC at the first use as 51%, 52% and 54% for CBHERD, RBBR, and CH GREEN dyes after 24 hours of incubation at 30°C, 150 rpm, respectively. These values were 11%, 21%, and 20% for dry forms of BC at the end of the first use. At the same time, the structure and morphology of bacterial cellulose were determined by SEM, XRD, and FTIR analysis. [ABSTRACT FROM AUTHOR]

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In this study, a novel immobilization support for laccase was developed to enhance enzyme stability, efficiency and reusability. Firstly, Fe 3 O 4 magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS). The support was characterized using several methods. Afterward, laccase was attached to the surface of functional support. The biochemical properties of the immobilized laccase were comprehensively investigated. It was observed that immobilized laccase achieved maximum activity at pH 4.0 and the optimum temperature was found to be 50 °C. After storage at +4 °C and ~25 °C for 4 weeks, the residual activity of the immobilized laccase was 87% and 80% of its initial activity, respectively. At 55 °C, the activity of immobilized laccase decreased to 73.4% in 180 min and after reused 20 times, the relative activity of immobilized laccase still was approximately 50% of its initial activity. Moreover, the textile dye (Reactive Blue 171 and Acid Blue 74) decolorization activity of immobilized laccase was also tested and it showed long-term textile dye decolorization activity. These results are promising for the use of laccase in industrial and biotechnological applications. Therefore, this functionalized magnetic hybrid composite might be used to immobilize laccase, an industrially important enzyme. • Fe 3 O 4 -TCS hybrid composite was synthesized for laccase immobilization. • Immobilized laccase showed high stability in a wider temperature and pH range. • The immobilized laccase exhibited well reusability after reused 20 times. • In addition, immobilized laccase showed long-term textile dye decolorization activity. [ABSTRACT FROM AUTHOR]