1. Oral administration of taurine improves experimental pancreatic fibrosis.
- Author
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Shirahige A, Mizushima T, Matsushita K, Sawa K, Ochi K, Ichimura M, Tanioka H, Shinji T, Koide N, and Tanimoto M
- Subjects
- Administration, Oral, Animals, Collagen Type I antagonists & inhibitors, Diet, Enzyme Inhibitors administration & dosage, Enzyme Inhibitors pharmacology, Fibrosis, Interleukin-2 metabolism, Interleukin-6 metabolism, Male, Matrix Metalloproteinase Inhibitors, Organotin Compounds, Pancreas metabolism, Pancreatitis chemically induced, Pancreatitis drug therapy, Rats, Rats, Wistar, Taurine administration & dosage, Taurine pharmacology, Transforming Growth Factor beta1 antagonists & inhibitors, Up-Regulation, Pancreas drug effects, Pancreas pathology, Pancreatitis pathology, Taurine analogs & derivatives
- Abstract
Background and Aim: The mechanism of pancreatic fibrosis is unclear. Taurine is used in the clinical treatment of a wide variety of diseases, but its effect on improving pancreatic fibrosis is unknown. We examined whether a diet with added taurine improves pancreatic fibrosis induced by dibutyltin dichloride (DBTC) in an experimental chronic pancreatitis rat model. In addition, we examined the influence of taurine on pancreatic stellate cells., Methods: Pancreatic fibrosis was induced by DBTC. Rats were fed a taurine-containing diet or a normal diet and were killed at 4 weeks. Pancreatic stellate cells were isolated from male Wistar rats. Cultured pancreatic stellate cells were incubated with or without taurine chloramine. Type I collagen and transforming growth factor-beta1 secretion was evaluated by ELISA, and matrix metalloproteinase activity was assessed by gelatin zymography. Interleukin-6, interleukin-2, and transforming growth factor-beta1 levels in the supernatants of pancreatic tissue homogenates were measured., Results: Pancreatic fibrosis induced by DBTC was improved remarkably by the oral administration of the taurine-containing diet. Taurine chloramine decreased type I collagen, transforming growth factor-beta1, and matrix metalloproteinases 2 of the pancreatic stellate cell culture supernatant. Increased interleukin-6 and decreased interleukin-2 were found in the supernatants of the pancreatic tissue homogenates of DBTC-induced pancreatitis rats compared with other groups., Conclusion: The oral administration of taurine improves pancreatic fibrosis. Taurine chloramine inhibits transforming growth factor-beta1 produced from activated pancreatic stellate cells and improves pancreatic fibrosis.
- Published
- 2008
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