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Tannic acid (TA) is a polyphenol with antioxidant properties present in various plants. In this study, we explored the protective effect of TA against ovarian oxidative stress in Brandt's voles and its underlying mechanism. At various doses, 3-nitropropionic acid (3-NPA) was intraperitoneally injected into Brandt's voles to simulate ovarian oxidative stress. Thereafter, various doses of TA were intragastrically administered to examine the protective effect of TA against 3-NPA-induced ovarian damage. Changes in inflammation, autophagy, apoptosis, and oxidative stress-related factors were investigated through various biochemical and histological techniques. Ovarian oxidative stress was successfully induced by the intraperitoneal administration of 12.5 mg/kg 3-NPA for 18 days. As a result, the ovarian coefficient decreased and ovarian tissue fibrosis was induced. TA treatment effectively alleviated the increase in luteinizing hormone and follicle-stimulating hormone levels; the decrease in estradiol, progesterone, and anti-Müllerian hormone levels; and the decline in fertility induced by 3-NPA. Compared to that in the 3-NPA group, TA decreased the expression of autophagy-related proteins beclin-1 and LC3, as well as the level of apoptosis. It also activated the AKT/mTOR signaling pathway, downregulated PTEN and p-NF-κB expression, and upregulated Nrf2 expression. In conclusion, our findings indicate that TA could inhibit autophagy via the regulation of AKT/mTOR signaling, suppressing oxidative damage and inflammatory responses through Nrf2 to alleviate 3-NPA-induced ovarian damage. Collectively, the current findings highlight the protective effects of TA in Brandt's vole, where it promotes the maintenance of normal ovarian function. [ABSTRACT FROM AUTHOR]

Simple Summary: Long-term evolution has resulted in plants developing defense mechanisms against herbivores, including physical defenses (such as hard outer skin, needles, thorns, etc.) and chemical defenses (such as toxic plant secondary metabolites, etc.) that interfere with animal eating, digestion, and metabolism, thus restricting animals' food choices. In this study, we investigated the effects of dietary tannic acid on the digestion and mucosal barrier function of Brandt's voles. The results revealed that plant tannins are a very powerful deterrent for Brandt's voles. This research advances our broad knowledge of interactions between plants and herbivores, as well as the ecological significance of plant secondary metabolites. Brandt's vole (Lasiopodomys brandtii) is a small herbivorous mammal that feeds on plants rich in secondary metabolites (PSMs), including tannins. However, plant defense mechanisms against herbivory by Brandt's voles are not clearly established. This study aimed to investigate the effects of dietary tannic acid (TA) on the growth performance, intestinal morphology, digestive enzyme activities, cecal fermentation, intestinal barrier function, and gut microbiota in Brandt's voles. The results showed that TA significantly hindered body weight gain, reduced daily food intake, changed the intestinal morphology, reduced digestive enzyme activity, and increased the serum zonulin levels (p < 0.05). The number of intestinal goblet and mast cells and the levels of serum cytokines and immunoglobulins (IgA, IgG, TNF-α, IL-6, and duodenal SlgA) were all reduced by TA (p < 0.05). Moreover, TA altered β-diversity in the colonic microbial community (p < 0.05). In conclusion, the results indicate that TA could damage the intestinal function of Brandt's voles by altering their intestinal morphology, decreasing digestive ability and intestinal barrier function, and altering microbiota composition. Our study investigated the effects of natural PSMs on the intestinal function of wildlife and improved our general understanding of plant–herbivore interactions and the ecological role of PSMs. [ABSTRACT FROM AUTHOR]

• Tannic acid treatment increased the adiponectin level of Brandt's voles. • Tannic acid promotes testicular glucose uptake by activating AMPK/GLUT1 pathway. • Tannic acid promote testicular development by regulating antioxidant and inflammatory cytokine levels. Adiponectin regulates steroid production and influences gonadal development. This study examined the effects of tannic acid (TA) on the adiponectin levels and gonads of male Brandt's voles. Male Brandt's voles aged 90 d were randomly separated into three groups: a control group (provided distilled water), a group given 600 mg∙kg-1 TA, and a group that received 1200 mg∙kg-1 TA (continuous gavage for 18 d). In this study, we examined the effects of TA on the adiponectin, antioxidant, and inflammatory levels in the testes. Furthermore, we examined the expression of important regulatory elements that influence adiponectin expression and glucose utilisation. In addition, the body weight, reproductive organ weight, and testicular shape were assessed. Our study observed that TA treatment increased serum adiponectin levels, DsbA-L and Ero1-Lα transcription levels, and AdipoR1, AMPK, GLUT1, and MCT4 expression levels in testicular tissue. TA enhanced pyruvate and lactic acid levels in the testicular tissue, boosted catalase activity, and reduced MDA concentrations. TA reduced the release of inflammatory factors in the testicular tissues of male Brandt's voles. TA increased the inner diameter of the seminiferous tubules. In conclusion, TA appears to stimulate adiponectin secretion and gonadal growth in male Brandt's voles while acting as an antioxidant and anti-inflammatory agent. [ABSTRACT FROM AUTHOR]

It is well known that tannins can influence the reproduction of animals, but there is little research published to elucidate the mechanics of this phenomenon. The purpose of this study was to investigate the role of antioxidation in the influence of tannic acid on the ovarian development of Brandt's voles (Lasiopodomys brandtii), which is a species of prairie animal that feeds on plants containing tannins. Postnatal 4-week-old female Brandt's voles were treated with 0 (control), 0.3% (low dose), or 0.6% (high dose) tannic acid for 4 or 9 weeks (i.e., when they reached puberty [8 weeks] or sexual maturity [13 weeks], respectively). The results showed that in both adolescent and adult Brandt's voles, firstly, treatment with tannic acid produced a higher ovary coefficient (ratio of the weight of ovaries to body weight), a greater proportion of mature follicles, and an increased follicular diameter. Secondly, tannic acid increased the serum contents of luteinizing hormone, follicle-stimulating hormone, and serum estradiol. Thirdly, tannic acid elevated the levels of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase) and reduced the level of malondialdehyde. Therefore, it is suggested that tannic acid may promote the ovarian development of female Brant's voles by enhancing their antioxidant capacity. [ABSTRACT FROM AUTHOR]

Tannic acid (TA) is one of the important plant secondary metabolites existed in the Brandt's vole's daily food. Its anti-nutritional and anti-oxidant effects have been previously reported. However, investigations about the microbial communities and alterations of gut microbiota in response to dietary TA have not been documented in Brandt's voles. To characterize the effects of dietary TA on the abundance and diversity of caecal microbes, 18 adult Brandt's voles were randomly assigned to 3 groups, where they were continuously fed with commercial rodent diet containing 0%, 3%, and 6% (w/w) TA for three weeks. The luminal contents of the caeca were collected for DNA extraction and high throughput sequencing was applied using an Illumina MiSeq. Significant differences in alpha diversity indices were detected between male and female voles. Female voles were significantly higher in Chao1, observed species, and the Shannon and Simpson indices than the males. The composition of bacterial community in the Brandt's voles was significantly affected by the administration of dietary TA. Gender, as well as the interaction between gender and TA played important roles in determining the bacterial structure. Female voles were observed to be more vulnerable than the males to the influence of dietary TA. This study was the first non-culture based microbiota analysis that revealed the caecal microbiota compositions in Brandt's voles. Our results provided preliminary data about sex-associated differences in the response of the caecal microbiota to dietary TA in Brandt's voles. [ABSTRACT FROM AUTHOR]