1. Development of a next-generation sequencing protocol for the canine T cell receptor beta chain repertoire.
- Author
-
Zuleger CL, Schwartz RW, Ong IM, Newton MA, Vail DM, and Albertini MR
- Subjects
- Dogs, Animals, Mice, High-Throughput Nucleotide Sequencing veterinary, Receptors, Antigen, T-Cell, alpha-beta genetics, T-Lymphocytes
- Abstract
Profiling the T cell receptor (TCR) repertoire using next-generation sequencing has become common in both human and translational research. Companion dogs with spontaneous tumors, including canine melanoma, share several features, e.g., natural occurrence, shared environmental exposures, natural outbred population, and immunocompetence. T cells play an important role in the adaptive immune system by recognizing specific antigens via a surface TCR. As such, understanding the canine T cell response to vaccines, cancer, immunotherapies, and infectious diseases is critically important for both dog and human health. Off-the-shelf commercial reagents, kits and services are readily available for human, non-human primate, and mouse in this context. However, these resources are limited for the canine. In this study, we present a cost-effective protocol for analysis of canine TCR beta chain genes. Workflow can be accomplished in 1-2 days starting with total RNA and resulting in libraries ready for sequencing on Illumina platforms., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier B.V.)
- Published
- 2024
- Full Text
- View/download PDF