1. Dendritic cells transfected with a polyepitope DNA construct stimulate an antitumor cytotoxic response in various tumors.
- Author
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Kurilin, Vasily, Kulikova, Ekaterina, Shevchenko, Julia, Lopatnikova, Julia, Obleukhova, Irina, Khantakova, Julia, Maksyutov, Amir, Kuznetsova, Maria, Khristin, Alexander, Kiryshina, Natalya, Kozlov, Vadim, Sidorov, Sergey, Sokolov, Andrey, Vitsin, Alexander, Shiku, Hiroshi, and Sennikov, Sergey
- Subjects
DENDRITIC cells ,CYTOTOXIC T cells ,NON-small-cell lung carcinoma ,DNA - Abstract
Dendritic cells (DCs) loaded with tumor-associated antigens (TAAs) are known to be crucial for the antitumor response and are still included in various treatment regimens in cancer immunotherapy research. In the present study, a cell-based protocol was evaluated, involving the use of original DNA constructs encoding the wide range of TAA epitopes expressed on different epithelial cancers. The constructs were transfected into in vitro-generated DCs of patients with various types of cancer, including breast, colorectal and non-small cell lung cancer. The direct cytotoxicity assay of effector cells, activated with the transfected DCs, revealed a significant increase in cytotoxicity against autologous tumor cells. The use of DNA constructs encoding a large number of TAAs for insertion into DCs in vitro, aiming to activate a T-cell response may prove to be a reliable and unified approach for immunotherapy and for the prevention of relapse in patients with epithelial cancers. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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