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Highlights: [•] Two novel introns are inserted in AGT from selected ray-finned fishes. [•] AGT is conserved on same syntenic fragment from 500 MY. [•] AGT became non-inhibitory from inhibitory serpin via RCL mutations. [•] AGT has 690 variants in 1092 human genomes from 14 populations. [•] 121 missense variants identified in human AGT protein with 32 deleterious mutants. [ABSTRACT FROM AUTHOR]

Heparin cofactor II (HCII) belongs to serpin superfamily and it acts as a thrombin inhibitor in the coagulation cascade, in a glycosaminoglycan-dependent pathway using the release of a sequestered hirudin-like N-terminal tail for interaction with thrombin. This serpin belongs to multiple member group V2 of vertebrate serpin classification. However, there is no comprehensive study illustrating the exact phylogenetic history of HCII, to date. Herein, we explored phylogenetic traits of HCII genes. Structures of HCII gene from selected ray-finned fishes and lamprey varied in exon I and II with insertions of novel introns of which one in core domain for ray-finned fishes in exon II at the position 241c. We found HCII remain nested in the largest intron of phosphatidylinositol (PI) 4-kinase (PIK4CA) gene (genetic variants of this gene cause schizophrenia) at the origin of vertebrates, dated about 500 MY old. We found that sequence features such as two acidic repeats (AR1-II), GAG-binding helix-D, three serpin motifs and inhibitory reactive center loop (RCL) of HCII protein are highly conserved in 55 vertebrates analyzed. We identified 985 HCII variants by analysis of 1092 human genomes with top three variation classes belongs to SNPs (84.3%), insertion (7.1%) and deletion (5.0%). We identified 37 deleterious mutations in the human HCII protein and we have described these mutations in relation to HCII sequence-structure-function relationships. These understandings may have clinical and medical importance as well. [ABSTRACT FROM AUTHOR]

C1 inhibitor (C1IN) is a multi-facet serine protease inhibitor in the plasma cascades, inhibiting several proteases, notably, regulates both complement and contact system activation. Despite huge advancements in the understanding of C1IN based on biochemical properties and its roles in the plasma cascades, the phylogenetic history of C1IN remains uncharacterized. To date, there is no comprehensive study illustrating the phylogenetic history of C1IN. Herein, we explored phylogenetic history of C1IN gene in vertebrates. Fishes have C1IN with two immunoglobulin like domains attached in the N-terminal region. The RCL regions of CIIN from fishes and tetrapod genomes have variations at the positions P2 and P1'. Gene structures of C1IN gene from selected ray-finned fishes varied in the Ig domain region with creation of novel intron splitting exon Im2 into Im2a and Im2b. This intron is limited to ray-finned fishes with genome size reduced below 1 Gb. Hence, we suggest that genome compaction and associated double-strand break repairs are behind this intron gain. This study reveals the evolutionary history of C1IN and confirmed that this gene remains the same locus for ~450 MY in 52 vertebrates analysed, but it is not found in frogs and lampreys. [ABSTRACT FROM AUTHOR]

Highlights: [•] ATIII possesses additional intron 262c is maintained in ray-finned fishes, but lost in other vertebrates. [•] ATIII is maintained in same genomic loci with few changes in fishes. [•] ATIII has 1997 variants in 1092 human genomes from 14 populations. [•] Three top variation classes are 76.2% SNPs, 11.8% deletions and 8.1% insertions. [•] There are 63 missense variants of human ATIII causes change in amino acids. [ABSTRACT FROM AUTHOR]