10 results on '"Liqing Sun"'
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2. Study of both fingerprint and high wavenumber Raman spectroscopy of pathological nasopharyngeal tissues
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Meizhen Huang, Rong Chen, Maowen Chen, Haishan Zeng, Wei Huang, Liqing Sun, Shanshan Wu, Yongzeng Li, Zhihong Xu, and Shaohua Huang
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Chemistry ,technology, industry, and agriculture ,Analytical chemistry ,medicine.disease ,Intensity ratio ,symbols.namesake ,Nuclear magnetic resonance ,Nasopharyngeal carcinoma ,Fingerprint ,Raman band ,symbols ,medicine ,General Materials Science ,Multivariate statistical ,Raman spectroscopy ,Spectroscopy ,Nasopharyngeal cancer - Abstract
High wavenumber (HW) Raman spectroscopy has weaker fluorescence background compared with fingerprint (FP) region. This study aims to evaluate the discrimination feasibility of nasopharyngeal non-cancerous and nasopharyngeal cancer (NPC) tissue with both FP and HW Raman spectroscopy. HW Raman spectra of nasopharyngeal tissue were obtained for the first time. Raman spectra were collected to differentiate nasopharyngeal non-cancerous (n = 37) from NPC (n = 41) tissues in FP (800–1800cm−1), HW (2700–3100cm−1), and integrated FP/HW region. First, to assess the utility of this method, the averaged Raman spectral intensities and intensity ratios of corresponding Raman bands were analyzed in HW and FP regions, respectively. The results show that intensities as well as the ratios of specific Raman peaks might be helpful in distinguishing nasopharyngeal non-cancerous from NPC tissue with the HW Raman spectroscopy, as with FP Raman reported before. The multivariate statistical method based on the combination of principal component analysis–liner discriminant analysis (PCA-LDA), together with leave-one-patient-out, cross-validation diagnostic algorithm, was used for discriminating nasopharyngeal non-cancerous from NPC tissue, generating sensitivities of 87.8%, 85.4%, and 95.1% and specificities of 86.5%, 91.9%, and 89.2%, respectively, with Raman spectroscopy in the FP, HW, and integrated FP/HW regions. The posterior probability of classification results and receiver operating characteristic curves were utilized to evaluate the discrimination of PCA-LDA algorithm, verifying that HW Raman spectroscopy has a positive effect on the differentiation for the diagnosis of NPC tissue by integrated FP/HW Raman spectroscopy. What's more, the potential of Raman spectroscopy used for differentiating different pathology NPC tissues was also discussed. The results demonstrate that both FP and HW Raman spectroscopy have the potential for diagnosis and detection in early nasopharyngeal carcinoma, and HW Raman spectroscopy may improve the discrimination of NPC tissue compared with FP region alone, providing a promising diagnostic tool for the diagnosis of NPC tissue. Copyright © 2015 John Wiley & Sons, Ltd.
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- 2015
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3. Human tissue analysis based on Raman spectroscopy for nasopharyngeal cancer detection
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Liqing Sun, Xueliang Lin, Zhihong Xu, Qiong wu, Xiaosong Ge, and Duo Lin
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symbols.namesake ,Pathology ,medicine.medical_specialty ,business.industry ,symbols ,Medicine ,business ,Raman spectroscopy ,Raman scattering ,Nasopharyngeal cancer ,Micro raman spectroscopy - Abstract
This article briefly reviews the applications of Raman spectroscopy technology for nasopharyngeal cancer tissue detection, especially presenting the corresponding work of our group in Fujian normal university, and discussing the perspective of this field.
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- 2017
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4. Comparative analysis of diagnostic efficiency of confocal micro-Raman spectroscopy and surface-enhanced Raman spectroscopy for nasopharyngeal carcinoma detection based on tissue smears method
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Xiaosong Ge, Xueliang Lin, Liqing Sun, Duo Lin, Wei Huang, and Zhihong Xu
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inorganic chemicals ,Materials science ,Confocal ,technology, industry, and agriculture ,macromolecular substances ,Surface-enhanced Raman spectroscopy ,medicine.disease ,Micro raman spectroscopy ,symbols.namesake ,Nuclear magnetic resonance ,Nasopharyngeal carcinoma ,medicine ,symbols ,Spectroscopy ,Raman spectroscopy ,Raman scattering - Abstract
Raman spectroscopy is a practice tool for tissue detection. This work demonstrated that confocal micro-Raman spectroscopy (CMRS) and surface-enhanced Raman spectroscopy (SERS) have promising exploratory potential for the detection of nasopharyngeal carcinoma (NPC) based on nasopharyngeal swab smear.
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- 2016
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5. Surface-enhanced Raman scattering spectroscopy for potential noninvasive nasopharyngeal cancer detection
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Liqing Sun, Jianji Pan, Buhong Li, Juqiang Lin, Rong Chen, Guannan Chen, Haishan Zeng, Shangyuan Feng, Chao Li, Shaojun Lin, and Zufang Huang
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symbols.namesake ,Materials science ,Nuclear magnetic resonance ,symbols ,General Materials Science ,Raman scattering spectroscopy ,Raman spectroscopy ,Spectroscopy ,Nasopharyngeal cancer - Published
- 2011
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6. Using micro-Raman spectroscopy for nasopharyngeal cancerous tissue detection
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Maowen Chen, Jia Lin, Liqing Sun, Wei Huang, Gang Cao, Rong Chen, and Shanshan Wu
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CHRONIC INFLAMMATIONS ,Chemistry ,Early detection ,medicine.disease ,Lymphoma ,Micro raman spectroscopy ,symbols.namesake ,Nuclear magnetic resonance ,Raman band ,medicine ,symbols ,Tissue diagnosis ,Raman spectroscopy ,Spectroscopy - Abstract
Micro-Raman spectroscopy is widely used for non-invasive tissue diagnosis and detection, as it provides detailed information about biomolecular composition, structure, and interaction of tissue. In this work, micro-Raman spectroscopy was used to investigate non-cancerous and cancerous nasopharyngeal tissues. The obtained nasopharyngeal tissue samples in vitro are divided into two groups: cancerous (n=12, undifferentiated non-keratinizing carcinomas) and non-cancerous (n=10, 7 chronic inflammations, 2 lymphomas and 1 lymphocytosis). Firstly, we analyzed the Raman spectra in the fingerprint (FP, 400-1800cm-1) region acquired. Preliminary results showed that there are some spectral differences in different pathological conditions. Furthermore, Raman spectra from cancerous and non-cancerous nasopharyngeal tissue in the high wavenumber region (HW, 2800-3100cm-1) were also reported for the first time. After detailed analysis, we achieved significant differences in Raman bands at 2854, 2874, 2934, and 3067cm-1 between cancerous and non-cancerous nasopharyngeal tissues. This study demonstrates that both fingerprint and high wavenumber regions of micro-Raman spectroscopy have the potential for the early detection of nasopharyngeal carcinomas.
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- 2014
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7. Immunoassay for CEA using the novel probe-labeled Ag nanoparticles based on surface-enhanced Raman scattering
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Liqing Sun, Yanping Chen, Rong Chen, Shangyuan Feng, Xiong-wei Zheng, Gang Chen, Gangqin Xi, Xiaoqian Lin, Jinping Lei, Haishan Zeng, and Lin Ou
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Absorption spectroscopy ,Chemistry ,Nanoparticle ,Nanotechnology ,biochemical phenomena, metabolism, and nutrition ,Surface-enhanced Raman spectroscopy ,Photochemistry ,law.invention ,symbols.namesake ,chemistry.chemical_compound ,law ,symbols ,bacteria ,Crystal violet ,Absorption (chemistry) ,Electron microscope ,Raman spectroscopy ,Raman scattering - Abstract
In this study, we report the Ag nanoparticles aggregated in the process of the labeling that use the crystal violet as the Raman probe. After this process- immune nanoparticles aggregate with high SERS sensitivity and biological specificity are created. We track and characterize the preparation by employing UV-Vi absorption spectra, Transmission Electron Microscope (TEM) and SERS spectra. With the increase of crystal violet, the aggregate of the Ag nanoparticles also increase, while the intensity of absorption peaks decrease. When the concentration of crystal violet reaches 1.0×10 -4 mol/L, new peaks were found in the long wavelengths, and with the increase of the crystal violet, the intensity of the new peaks increase as well. We observe from the TEM, that with the increase of crystal violet, the aggregation degree of the Ag nanoparticles also increase and then they unite together. The SERS activity of the aggregates was directly related to the aggregation degree. Detecting the SERS activity of Ag NPs aggregates labeled with different amount of crystal violet, we found that with the increase of the crystal violet, the SERS signals of NPs aggregates enhanced. However, when the amount of crystal violet exceeded 25μL in 1mL colloidal silver, the Ag NPs occurred agglomeration and thereafter the next preparation of immune-label aggregates was hindered. Whereas, the probe labelled with 12μL crystal violet exhibited a better stability, stronger SERS activity and higher biological specificity, and it may accomplish a highly efficient SERS-based immunoassay. This immune probe was applied for detecting the expression of carcinoembryonic antigen (CEA) in the colon cancer tissue slice. Results show that appropriate immune aggregates labelled with optimum quantity of crystal violet present high stability, strong SERS activity and good immune specificity, which are expected to be applied in the analysis of the protein expression in the tissue section and promising for developing into a clinical tool for diagnosis.
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- 2012
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8. Diagnostic potential for gold nanoparticle-based surface-enhanced Raman spectroscopy to provide colorectal cancer screening using blood serum sample
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Duo Lin, Liqing Sun, Jianji Pan, Yanping Chen, Rong Chen, Shangyuan Feng, and Juqiang Lin
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Chemistry ,Colorectal cancer ,Nanoparticle ,Cancer ,Nanotechnology ,Surface-enhanced Raman spectroscopy ,Control subjects ,medicine.disease ,symbols.namesake ,Nuclear magnetic resonance ,Blood serum ,Colorectal cancer screening ,symbols ,medicine ,Raman spectroscopy - Abstract
Surface-enhanced Raman spectroscopy (SERS) is a vibrational spectroscopic technique that is capable of probing the biomolecular changes associated with diseased transformation. The objective of our study was to explore gold nanoparticle based SERS to obtain blood serum biochemical information for non-invasive colorectal cancer detection. SERS measurements were performed on two groups of blood serum samples: one group from patients (n = 38) with pathologically confirmed colorectal cancer and the other group from healthy volunteers (control subjects, n = 45). Tentative assignments of the Raman bands in the measured SERS spectra suggested interesting cancer specific biomolecular changes, including an increase in the relative amounts of nucleic acid, a decrease in the percentage of saccharide and proteins contents in the blood serum of colorectal cancer patients as compared to that of healthy subjects. Principal component analysis (PCA) of the measured SERS spectra separated the spectral features of the two groups into two distinct clusters with little overlaps. Linear discriminate analysis (LDA) based on the PCA generated features differentiated the nasopharyngeal cancer SERS spectra from normal SERS spectra with high sensitivity (97.4%) and specificity (100%). The results from this exploratory study demonstrated that gold nanoparticle based SERS serum analysis combined with PCA-LDA has tremendous potential for the non-invasive detection of colorectal cancers.
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- 2012
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9. Surfaced-enhanced Raman spectroscopy of nasopharyngeal carcinoma cell using gol nanoparticles
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Weiwei Chen, Ying Su, Yongzeng Li, Liqing Sun, Jianji Pan, Hao Huang, Rong Chen, and Shangyuan Feng
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Materials science ,Analytical chemistry ,Nanoparticle ,Surface-enhanced Raman spectroscopy ,Photochemistry ,medicine.disease ,symbols.namesake ,Nasopharyngeal carcinoma ,Colloidal gold ,Cell culture ,medicine ,symbols ,Spectroscopy ,Raman spectroscopy ,Raman scattering - Abstract
The surface-enhanced Raman scattering (SERS) spectroscopy and normal Raman spectroscopy of single living human nasopharyngeal carcinoma cells(CNE-1) were tested and analyzed by gold nanoparticles incubation into cells. The characteristic Raman bands in the SERS spectra of living cells were tentatively assigned. Six obvious Raman bands (718 A 1001 A 1123 A 1336 A 1446 A 1660 cm -1 A were observed in the normal Raman sp ectroscopy of living CNE-1 cells. Colloidal gold nanopar ticles that were introduced inside cells resulted in strongly enhan ced Raman signals of the native chemical constituents of the cells, and over twenty SERS Raman bands were obse rved in the SERS spectroscopy of living CNE-1 cells. Four vibrations of the DNA backbone (1026, 1097, 1336 and 1585 cm -1 A confirmed that some gold nanoparticles were able to enter the nucleus. The results showed that, based on colloidal gold, the SERS spectroscopy might provide a sensitive and structurally selective detecting me thod for native chemicals insi de a cell, such as DNA and phenylalanine. Keywords: Surface-enhanced Raman spectroscopy, gold co lloids, human nasopharyngeal carcinoma cell line.
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- 2010
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10. Surface-enhanced Raman scattering spectroscopy for potential noninvasive nasopharyngeal cancer detection
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Buhong Li, Zufang Huang, Chao Li, Shaojun Lin, Liqing Sun, Rong Chen, Jianji Pan, Juqiang Lin, Shangyuan Feng, Haishan Zeng, and Guannan Chen
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Chemistry ,Cancer ,Raman scattering spectroscopy ,medicine.disease ,Blood proteins ,Silver nanoparticle ,symbols.namesake ,Nuclear magnetic resonance ,symbols ,medicine ,General Materials Science ,Raman spectroscopy ,Spectroscopy ,Raman scattering ,Nasopharyngeal cancer - Abstract
Combining membrane electrophoresis with surface-enhanced Raman scattering (SERS) spectroscopy, the serum proteins were first purified and then mixed with silver nanoparticles to perform SERS spectral analysis. Therefore, the spectral signatures were enhanced to high-fidelity SERS signatures because of the purification procedure of the first step. We used the method to analyze blood plasma samples from nasopharyngeal cancer patients (n = 43) and healthy volunteers (n = 33) for cancer detection. Principle component analysis of the SERS spectra revealed that the data points for the cancer group and the normal group form distinct, completely separated clusters with no overlap. Therefore, the nasopharyngeal cancer group can be unambiguously discriminated from the normal group, i.e., with both diagnostic sensitivity and specificity of 100%. These results are very promising for developing a label-free, noninvasive, and reliable clinical tool for rapid cancer detection and screening. Copyright © 2011 John Wiley & Sons, Ltd.
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- 2013
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