1. Insight into the role of Streptococcus suis zinc metalloprotease C from the new serotype causing meningitis in piglets.
- Author
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Gu Q, He P, Bai Q, Zhong X, Zhang Y, Ma J, Yao H, and Pan Z
- Subjects
- Animals, Swine, Mice, Female, Virulence Factors metabolism, Virulence Factors genetics, Bacterial Proteins metabolism, Bacterial Proteins genetics, Humans, Matrix Metalloproteinase 9 metabolism, Matrix Metalloproteinase 9 genetics, Mice, Inbred BALB C, Metalloendopeptidases metabolism, Metalloendopeptidases genetics, Streptococcus suis pathogenicity, Streptococcus suis enzymology, Streptococcal Infections veterinary, Streptococcal Infections microbiology, Swine Diseases microbiology, Serogroup, Meningitis, Bacterial veterinary, Meningitis, Bacterial microbiology
- Abstract
Streptococcus suis (S. suis) is an important gram-positive pathogen and an emerging zoonotic pathogen that causes meningitis in swine and humans. Although several virulence factors have been characterized in S. suis, the underlying mechanisms of pathogenesis are not fully understood. In this study, we identified Zinc metalloproteinase C (ZmpC) probably as a critical virulence factor widely distributed in S. suis strains. ZmpC was identified as a critical facilitator in the development of bacterial meningitis, as evidenced by the detection of increased expression of TNF-α, IL-8, and matrix metalloprotease 9 (MMP-9). Subcellular localization analysis further revealed that ZmpC was localized to the cell wall surface and gelatin zymography analysis showed that ZmpC could cleave human MMP-9. Mice challenge demonstrated that ZmpC provided protection against S. suis CZ130302 (serotype Chz) and ZY05719 (serotype 2) infection. In conclusion, these results reveal that ZmpC plays an important role in promoting CZ130302 to cause mouse meningitis and may be a potential candidate for a S. suis CZ130302 vaccine., (© 2024. The Author(s).)
- Published
- 2024
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