1. A high-resolution comparative map of porcine chromosome 4 (SSC4).
- Author
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Ma JG, Chang TC, Yasue H, Farmer AD, Crow JA, Eyer K, Hiraiwa H, Shimogiri T, Meyers SN, Beever JE, Schook LB, Retzel EF, Beattie CW, and Liu WS
- Subjects
- Animals, Chromosomes, Artificial, Bacterial, Focal Adhesion Kinase 1 genetics, Humans, Likelihood Functions, Microsatellite Repeats genetics, Radiation Hybrid Mapping, Species Specificity, Synteny genetics, Chromosome Mapping methods, Chromosomes, Mammalian genetics, Gene Expression Profiling methods, Swine genetics
- Abstract
We used the IMNpRH2(12,000-rad) RH and IMpRH(7,000-rad) panels to integrate 2019 transcriptome (RNA-seq)-generated contigs with markers from the porcine genetic and radiation hybrid (RH) maps and bacterial artificial chromosome finger-printed contigs, into 1) parallel framework maps (LOD ≥ 10) on both panels for swine chromosome (SSC) 4, and 2) a high-resolution comparative map of SSC4, thus and human chromosomes (HSA) 1 and 8. A total of 573 loci were anchored and ordered on SSC4 closing gaps identified in the porcine sequence assembly Sscrofa9. Alignment of the SSC4 RH with the genetic map identified five microsatellites incorrectly mapped around the centromeric region in the genetic map. Further alignment of the RH and comparative maps with the genome sequence identified four additional regions of discrepancy that are also suggestive of errors in assembly, three of which were resolved through conserved synteny with blocks on HSA1 and HSA8., (© 2010 The Authors, Animal Genetics © 2010 Stichting International Foundation for Animal Genetics.)
- Published
- 2011
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