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Intestinal translocation is a key factor for determining bioavailability of nanoparticles (NPs) after oral uptake. Therefore, we evaluated threein vitrointestinal cell models of increasing complexity which might affect the translocation of NPs: a mono-culture (Caco-2 cells), a co-culture with mucus secreting HT29-MTX cells and a tri-culture with M-cells. Cell models were exposed to well characterized differently sized (50 and 100 nm) and charged (neutral, positively and negatively) polystyrene NPs. In addition, two types of negatively charged NPs with different surface chemistries were used. Size strongly affected the translocation of NPs, ranging up to 7.8% for the 50 nm NPs and 0.8% for the 100 nm NPs. Surface charge of NPs affected the translocation, however, surface chemistry seems more important, as the two types of negatively charged 50 nm NPs had an over 30-fold difference in translocation. Compared with the Caco-2 mono-culture, presence of mucus significantly reduced the translocation of neutral 50 nm NPs, but significantly increased the translocation of one type of negatively charged NPs. Incorporation of M-cells shifted the translocation rates for both NPs closer to those in the mono-culture model. The relative pattern of NP translocation in all three models was similar, but the absolute amounts of translocated NPs differed per model. We conclude that for comparing the relative translocation of different NPs, using one intestinal model is sufficient. To choose the most representative model for risk assessment,in vivoexperiments are now needed to determine thein vivotranslocation rates of the used NPs. [ABSTRACT FROM PUBLISHER]

This paper discusses the problem of thermocycling effect of light-curing polymer–ceramic composites. Cyclic thermal shocks were simulated in laboratory conditions. As a rule, these loads were supposed to reproduce the actual conditions of biomaterials exploitation. Periodically variable stresses occurring in dental restorations are associated with the wear of cold and hot foods and beverages. They lead to changes in the properties of composites, including the properties of the surface layer. The aim of the work was to assess the impact of cyclic hydrothermal interactions on the properties of the surface layer of composites relevant to the operational quality. Two commercial materials manufactured by the world's leading producer (3M ESPE)—Filtek Z550, Filtek Flow and two experimental, micro-hybrid and flow type composites marked Ex-mhyb(P) and Ex-flow(P), respectively. All tests were carried out before and after hydro-thermal cycles (flowing water thermocycling). Micro-hardness test using the Vickers method, indentation hardness, and resistance to tribological wear in a ball–disc system in sliding friction conditions were performed. In addition, observations of the surface layer of composites on the SEM (scanning electron microscope) were carried out. It was noticed that semi-liquid composites, containing a smaller amount of filler, retain higher stability of mechanical and tribological properties of the surface layer under cyclic hydro-thermal loads. Coefficient of friction of samples after hydro-thermal cycles increased for micro-hybrid materials and Filtek Flow (FFlow) composite. In the case of Ex-flow(P) material, the coefficient of friction decreased. The microhardness of composites also changed, the variability of this size depended on the type of material. Composites with a higher content of filler particles were characterized by greater variability of microhardness under the influence of thermocycles. The resistance to tribological wear also changed in a similar way. Composites containing higher volume fraction of inorganic filler showed higher tribological wear after thermocycling. The wear resistance of flow composites changed to a lesser extent, after thermocycling increased. The paper also showed that, in real kinematic nodes, the surface layer of light-curing ceramic–polymer composites is exposed to significant non-tribological (erosive, thermal, and chemical) defects that synergize with tribological ones. In slip pairs loaded dynamically, under mixed friction conditions, tribological wear of PMCCs (polymer matrix ceramic composites) is manifested by spalling (spalling of the material flakes, in particular the polymer phase) and pitting (crushing wear caused by wear products, in particular large filler particles or clusters, previously adhesively extracted). [ABSTRACT FROM AUTHOR]