1. Quantification of tumor necrosis factor-α and matrix metalloproteinases-3 in synovial fluid by a fiber-optic particle plasmon resonance sensor.
- Author
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Huang YC, Chiang CY, Li CH, Chang TC, Chiang CS, Chau LK, Huang KW, Wu CW, Wang SC, and Lyu SR
- Subjects
- Feasibility Studies, Humans, Osteoarthritis diagnosis, Biosensing Techniques methods, Matrix Metalloproteinase 3 analysis, Optical Fibers, Surface Plasmon Resonance methods, Synovial Fluid chemistry, Tumor Necrosis Factor-alpha analysis
- Abstract
The availability of techniques for sensitive detection of early stage osteoarthritis is critical for improving patient health. This study illustrates the feasibility of a fiber-optic particle plasmon resonance (FOPPR) sensor with gold nanoparticles on the unclad region of optical fiber probes for analysis of osteoarthritis biomarkers, tumor necrosis factor-α (TNF-α) and matrix metalloproteinases-3 (MMP-3). Results show that the sensor can achieve a refractive index resolution of 5.18 × 10⁻⁷ RIU and limits of detection for TNF-α and MMP-3 as low as 8.22 pg ml⁻¹ (0.48 pM) and 34.3 pg ml⁻¹ (1.56 pM), respectively. Additionally, the FOPPR sensor shows a good correlation in determining TNF-α and MMP-3 in synovial fluid with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method. Finally, given the FOPPR sensor's nature of being low-cost, label-free, highly sensitive, real-time, simple-to-operate, the FOPPR sensor could offer potential to monitor biomarkers of various diseases, and provide an ideal technical tool for point-of-care diagnostics.
- Published
- 2013
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