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This paper presents an electrochemical-surface plasmon resonance imaging (EC-SPRI) system, enabling the characterization of optical and electrical properties of cells, simultaneously. The developed surface plasmon resonance (SPR) imaging system was capable of imaging micro cavities with a dimension of 10 μm × 10 μm and differentiated glycerol solutions with a group of refractive indices (RIs). Furthermore, the EC-SPRI system was used to image A549 cells, suggesting corresponding RI and morphology changes during the cell death process. In the end, electrochemical and SPR methods were used in combination, recording oxidation peaks of A549 cells in the cyclic voltage curves and SPR response unit increase, simultaneously.

This paper presents a surface plasmon resonance (SPR) imaging system based on a low-cost, convenient poly(dimethylsiloxane) (PDMS) prism featured with a close contact with the gold film. Compared to conventional glass prism, both numerical simulations and experimental studies indicated a deeper but wider absorption peak with a higher coupling angle for the PDMS based prism. System repeatability was quantified by the cycled detection of helium and air, with the effect of the flow rate investigated. Furthermore, five types of gases (nitrogen, air, oxygen, hydrogen, and helium) were detected and differentiated by the SPR system, with a calculated sensitivity of 5 × 10(-6) RIU.

A novel and effective method for target cells capture and detection is developed in a label-free and real-time mode. The cultured cell line (TZMbl) expressed as CD4+ was used as the positive CD4+ T lymphocytes for target cells capture and detection experiments. The target cells were captured on various surfaces (such as glass, polydimethylsiloxane, and metal) based on a specific immunity method. The gold surface of the surface plasmon resonance (SPR) sensing chip modified with a nano denaturalised bovine serum albumin layer had the best capture capacity. A total of 90.9% positive CD4+ T lymphocytes were captured with a slow flow rate of 5 μl/min, whereas 84.1% of cells were kept with a fast flow rate of 2 ml/min. Furthermore, the captured positive cells on the SPR sensing chip were detected on-line by using author's home-made SPR biosensor. From the experimental results, 75 cells/μl could produce an obvious change in the SPR response signal, which holds great potential for target cells capture, reorganisation and further analysis of CD4+ T lymphocytes in human peripheral blood. [ABSTRACT FROM AUTHOR]