1. ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation
- Author
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Oracz, K., El-Maarouf Bouteau, H., Farrant, J.M., Cooper, K., Belghazi, Maya, Job, C., Job, D., Corbineau, F., Bailly, Cedric, ProdInra, Migration, Warsaw Agricultural University, Université Pierre et Marie Curie - Paris 6 (UPMC), University of the Western Cape (UWC), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Physiologie des plantes et des champignons lors de l'infection, Bayer Cropscience-Centre National de la Recherche Scientifique (CNRS), Bayer SAS, University of the Western Cape, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
reactive oxygen species ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,after-ripening ,sunflower ,ANALYSE DU PROTEOME ,[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics ,seed dormancy ,food and beverages ,carbonylation ,proteome analysis ,ComputingMilieux_MISCELLANEOUS - Abstract
At harvest, sunflower (Helianthus annuus L.) seeds are dormant and unable to germinate at temperatures below 15C. Seed storage in the dry state, known as after-ripening, is associated with an alleviation of embryonic dormancy allowing subsequent germination at suboptimal temperatures. To identify the process by which dormancy is broken during after-ripening, we focused on the role of reactive oxygen species (ROS) in this phenomenon. After-ripening entailed a progressive accumulation of ROS, namely superoxide anions and hydrogen peroxide, in cells of embryonic axes. This accumulation, which was investigated at the cellular level by electron microscopy, occurred concomitantly with lipid peroxidation and oxidation (carbonylation) of specific embryo proteins. Incubation of dormant seeds for 3 h in the presence of hydrogen cyanide (a compound that breaks dormancy) or methylviologen (a ROS-generating compound) also released dormancy and caused the oxidation of a specific set of embryo proteins. From these observations, we propose a novel mechanism for seed dormancy alleviation. This mechanism involves ROS production and targeted changes in protein carbonylation patterns.
- Published
- 2007
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