1. Sensitive Determination of Cobalt(II) Using a Spiro Fluorescein Hydrazide as a Chemiluminogenic Reagent
- Author
-
Xiao-Feng Yang, Hua Li, and Dong-Bing Wu
- Subjects
Detection limit ,Autoxidation ,Photochemistry ,Analytical Chemistry ,Catalysis ,law.invention ,chemistry.chemical_compound ,Sulfite ,chemistry ,law ,Reagent ,Basic solution ,Fluorescein ,Nuclear chemistry ,Chemiluminescence - Abstract
A novel chemiluminogenic reagent, refered to as spiro form fluorescein hydrazide, was synthesized, and its application to chemiluminescence (CL) determination of Co2+ is described. The method is based on the catalytic effect of Co2+ on the autoxidation of sulfite in basic solution to generate a series of powerful oxidative intermediates, which then oxidize colorless, nonfluoresent fluorescein hydrazide to generate strong CL emission. The CL signal is linearly related to the concentration of Co2+ in the range of 0.1–200 nM with a detection limit of 0.04 nM. The optimal conditions for the detection of Co2+ were evaluated, and the possible CL mechanism is discussed. The proposed method has been applied to the determination of Co2+ in pharmaceutical preparations and in soil samples.
- Published
- 2004
- Full Text
- View/download PDF