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Objective: To study the effect of diltiazem on monosodium glutamate induced histomorphological changes in the fallopian tube. Study Design: Randomized controlled trial. Material and Methods: In this experimental study 30 adult female Sprague Dawley rats of average weight of 500g were randomly divided into three groups A (control group), B and C with 10 rats in each. The experimental group B was given 0.08 mg/kg of monosodium glutamate (MSG) orally and experimental group C was given 0.08 mg/kg of MSG and 10 mg/kg of diltiazem in distilled water for 14 days. The control group (A) received only laboratory diet prepared at National Institute of Health (NIH) Islamabad with liberal water intake. Blood was taken from each animal through intracardiac route. The rats were sacrificed on 15th day of the experiment. The fallopian tubes were carefully dissected out and fixed in 10% formalin for routine histological procedures. Results: The fallopian tubes of the control group A showed normal histological features, a well-defined tubal wall consisted of three distinct layers. Oviducts of group B also showed three distinct layers. The cells forming the external serosal layer were flat with centrally located nuclei giving the appearance of simple. The cells forming the intermediate muscular layer were fusiform in appearance with centrally placed nuclei giving the appearance of smooth muscle cells. The cells in the inner mucosal layer were taller than broader with oval nuclei giving the appearance of simple columnar epithelium resting on the basement membrane. There were marked vacuolations appearing in the stroma cells. The blood vessels in stroma were dilated. The histological sections of oviducts of group C showed three layers: serosa, myosalpinx, endosalpinx the external serosal layer were flat with centrally located nuclei. At the inner side of this layer, there were lying irregularly scattered thin fibers, blood vessels and irregular shaped cells. The stromal cells were loosely arranged and in all animals of this group there was no vacuolations in stroma of this group. The mean serum estrogen level for group A was 83.53 ± 9.87 pmol/l. The mean serum estrogen level in group B was 136.97 ± 12.51 pmol/l. The mean serum estrogen level in group C was 64.79 ± 17.85 pmol/l. Conclusion: The findings obtained from our study after administration of MSG and diltiazem showed stromal vacuolations in the fallopian tube of rat leading to change in its functional aspect and impairing the female fertility while treatment with diltiazem prevents these effects.