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Men with diabetes have negative effects on reproduction that causes sexual dysfunction. Medicinal plants are non‐toxic and much safer than synthetic drugs because regular use of synthetic drugs shows long‐term side effects. Curcuma amada (Roxb) is a medicinal plant used in Ayurveda and Unani medicinal systems in India. The goal of this study is to rummage the potential efficiency of the most potent solvent fraction of effective extract of hydro‐methanol 60:40 of C. amada rhizome on male gonadal hypofunction in streptozotocin‐induced diabetic rat. Diabetes‐induced testicular hypofunction was evaluated by glycemic, spermiological, biochemical, genomic, flow cytometric, and histology of testicular tissue. The n‐hexane, chloroform, ethyl‐acetate, and n‐butanol solvent fractions of the said extract were administrated for 4 weeks at 10 mg dose/100 g body weight/day. Among all the used fractions, the ethyl‐acetate solvent fraction‐treated group showed maximum recovery in serum insulin (177.42%), sperm count (92.84%), sperm motility (97.15%), and serum testosterone (164.33%). The diabetic rats treated with ethyl‐acetate solvent fraction also exhibited the maximum resettlement in flow cytometric analysis of sperm viability (55.84%) and sperm mitochondrial integrity (149.79%), gene expression patterns of key markers for androgenesis (Δ5, 3β‐HSD 87.50%, and 17β‐HSD 74.66%) and apoptosis (Bax 44.63%, Bcl‐2 54.03%, and Caspase‐3 35.77%) along with testicular histology. The ethyl‐acetate fraction contains alkaloids, flavonoids, and polyphenols where all of these components are not present in other fractions, may be the most effective cause for the recovery of diabetes‐linked oxidative stress‐mediated testicular hypofunctions. Practical applications: Nowadays worldwide, the use of synthetic drugs are reduced due to their toxic effect. At present, synthetic drugs are replaced by several herbal drugs, the natural source of medicine which has many therapeutic values. C. amada has strong antioxidant activity due to the presence of bio‐active compound(s) that can able to manage streptozotocin‐induced diabetes linked to oxidative damage of male gonadal organs. Therefore, these bio‐active compound(s)‐containing said medicinal plant may use as a good source of antioxidative food in the food industry as nutraceuticals and in pharmaceutical industries for the development of the herbal drug to manage diabetes‐linked male gonadal hypofunctions. At present, WHO also gives emphasis for developing one drug‐multi‐disease therapy. From such a viewpoint, this active fraction‐containing phytomolecules may have corrective efficacy against diabetes as well as oxidative stress‐linked testicular complications. [ABSTRACT FROM AUTHOR]

Diabetes affects the reproductive system. This study was conducted to find out the potent dose of the hydro‐methanol 60:40 extract of Curcuma amada rhizomes for the management of diabetes‐induced testicular dysfunction in albino rats. The extract was administered at the doses of 10, 20, 40, and 80 mg/100 g body weight/day for 28 days. Oxidative stresses, reproductive parameters, histological, and gene expressions of the testicular tissue were assessed. Out of the doses used, the 20‐mg dose showed maximum recovery as the minimum dose (e.g., sperm motility 112.03%, testicular cholesterol 34.86%, Bax gene expression 49.77%), whereas 40‐ and 80‐mg doses did not vary statistically with each other (e.g., sperm motility 95.37% and 89.19%, testicular cholesterol 30.42% and 28.41%, Bax gene expression 47.33% and 46.18%, respectively) as well as with the 20‐mg dose. It may be concluded that the 20‐mg dose is the threshold dose for this purpose. Practical applications: The hydro‐methanol 60:40 extract of rhizomes of Curcuma amada has a strong antioxidant property that can manage diabetes‐induced oxidative injuries in testes which may raise a hope to the pharmaceutical industries to develop a herbal drug for diabetes‐linked testicular hypofunction management. [ABSTRACT FROM AUTHOR]