1. Genome Mining and Enzymatic Total Biosynthesis of Purincyclamide.
- Author
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Shi J, Xu X, Zhao EJ, Zhang B, Li W, Zhao Y, Jiao RH, Tan RX, and Ge HM
- Subjects
- Molecular Structure, Peptide Synthases chemistry, Peptide Synthases genetics, Purines chemistry, Purines isolation & purification, Sulfonylurea Compounds chemistry, Sulfonylurea Compounds isolation & purification, Peptide Synthases metabolism, Purines biosynthesis, Streptomyces enzymology, Sulfonylurea Compounds metabolism
- Abstract
A cyclodipeptide synthase-containing gene cluster ( pcm ) was identified in Streptomyces chrestomyceticus NA4264 through genome mining. Heterologous expression of the cryptic pcm gene cluster in Streptomyces albus led to the production of a new highly modified cyclodipeptide named purincyclamide ( 1 ). Bioinformatic analysis and gene knockout experiments revealed the biosynthetic pathway. The production of 1 was achieved through a one-pot enzymatic reaction.
- Published
- 2019
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