1. A LAMP-based assay for the molecular detection of group B Streptococcus.
- Author
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Lemaire, Coralie, Cheminet, Mélinda, Duployez, Claire, Artus, Mathilde, Ballaa, Yassine, Devos, Laura, Plainvert, Céline, Poyart, Claire, Le Gall, Florence, Tazi, Asmaa, and Lanotte, Philippe
- Subjects
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STREPTOCOCCUS agalactiae , *NEONATAL infections , *STREPTOCOCCUS , *ANTIBIOTIC prophylaxis , *PREGNANT women , *STREPTOCOCCUS pneumoniae - Abstract
Purpose: Streptococcus agalactiae remains a major pathogen in human health, especially in neonatal infection. Detection in pregnant women is essential to initiate intrapartum antibiotic prophylaxis. This study compared the HiberGene loop-mediated isothermal amplification (LAMP) assay to culture, the reference method, for the detection of group B Streptococcus (GBS) in pregnant women. Methods: This was a prospective multicenter study conducted in four French hospitals. Three hundred fifty-four non-redundant routine care vaginal swabs were analyzed by both methods, LAMP assay and culture. Clinicians and patients were blinded to the results of the LAMP assay. Results: Three hundred thirty-seven samples presented concordant results, 15 presented discordant results, and 2 were invalid using the LAMP assay (excluded from the study). Compared to culture, the LAMP assay had a sensitivity of 87.7%, a specificity of 98%, a negative predictive value of 97.6%, and a positive predictive value of 89.3%. Conclusion: The HiberGene GBS LAMP assay is an easy test that possesses good performances compared with the reference method, culture. It could be used in case of emergency when a quick result is needed. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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