6 results on '"Ewbank, Ana Carolina"'
Search Results
2. Postmortem findings in Magellanic penguins (Spheniscus magellanicus) caught in a drift gillnet
- Author
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Ewbank, Ana Carolina, Sacristán, Carlos, Costa-Silva, Samira, Antonelli, Marzia, Lorenço, Janaina R., Nogueira, Guilherme A., Ebert, Mariana B., Kolesnikovas, Cristiane K. M., and Catão-Dias, José Luiz
- Published
- 2020
- Full Text
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3. Parafilaroidose pulmonar fatal em um lobo-marinho-subantártico (Arctocephalus tropicalis) de vida livre coinfectado com dois gammaherpesvírus e Sarcocystis sp
- Author
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Reisfeld,Laura, Sacristán,Carlos, Sánchez-Sarmiento,Angélica María, Costa-Silva,Samira, Díaz-Delgado,Josué, Groch,Kátia Regina, Marigo,Juliana, Ewbank,Ana Carolina, Favero,Cíntia Maria, Guerra,Juliana Mariotti, Réssio,Rodrigo Albergaria, Cremer,Marta Jussara, Esperón,Fernando, and Catão-Dias,José Luiz
- Subjects
stranding ,encalhe ,pneumonia ,Herpesvirus ,Herpesvírus ,infecção por protozoários ,Parafilaroides sp ,protozoan infection ,pinniped ,pinípede - Abstract
A juvenile subantarctic fur seal (Arctocephalus tropicalis) found dead in Santa Catarina state, southern Brazil, presented with disseminated verminous pneumonia due to Parafilaroides sp. A concomitant infection with two different gammaherpesviruses was identified by PCR in different tissues; one of them possibly a novel species (tentatively named Otariid herpesvirus 7). Sarcocystis sp. DNA was identified molecularly in skeletal muscle samples with intrasarcoplasmic bradyzoites and no apparent tissue response. All analyzed samples (mandibular, laryngeal, tracheal, and mesenteric lymph nodes, and lung) were PCR-negative for Brucella spp. The most likely cause of death was severe pulmonary parafilaroidiasis. The pathogenic role of the gammaherpesviruses in several of the tissues was not evident. This study describes the pathogenicity of Parafilaroides sp. in a subantarctic fur seal, widens the host range of herpesvirus in pinnipeds, and reports the first molecular identification of Sarcocystis sp. in this species. Resumo Um lobo-marinho-subantártico (Arctocephalus tropicalis) juvenil foi achado morto no Estado de Santa Catarina, sul do Brasil, apresentando pneumonia parasitária disseminada por Parafilaroides sp. Infecção concomitante por dois gammaherpesvírus diferentes foi identificada pela PCR em diversos tecidos, um desses herpesvírus possivelmente uma nova espécie (denominada provisoriamente Otariid herpesvirus 7). DNA de Sarcocystis sp. foi identificado molecularmente em amostras de músculo esquelético que apresentavam bradizoítos intra-sarcoplasmáticos sem aparente resposta tecidual. Todas as amostras analisadas (linfonodo mandibular, laríngeo, traqueal e mesentérico, e pulmão) pela PCR para Brucella spp. foram negativas. A causa mais provável da morte do animal foi parafilaroidose pulmonar severa. O papel patogénico dos gammaherpesvírus em vários tecidos não foi evidente. Este estudo descreve a patogenicidade de Parafilaroides sp. em um lobo-marinho-subantártico, amplia a variedade de hospedeiros de herpesvírus em pinípedes e reporta a primeira identificação molecular de Sarcocystis sp. para essa espécie.
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- 2019
4. Fatal pulmonary parafilaroidiasis in a free-ranging subantarctic fur seal (Arctocephalus tropicalis) coinfected with two gammaherpesviruses and Sarcocystis sp
- Author
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Reisfeld, Laura, Sacristán, Carlos, Sánchez-Sarmiento, Angélica María, Costa-Silva, Samira, Díaz-Delgado, Josué, Groch, Kátia Regina, Marigo, Juliana, Ewbank, Ana Carolina, Favero, Cíntia Maria, Guerra, Juliana Mariotti, Réssio, Rodrigo Albergaria, Cremer, Marta Jussara, Esperón, Fernando, and Catão-Dias, José Luiz
- Subjects
stranding ,encalhe ,pneumonia ,Herpesvirus ,Herpesvírus ,infecção por protozoários ,Parafilaroides sp ,protozoan infection ,pinniped ,pinípede - Abstract
A juvenile subantarctic fur seal (Arctocephalus tropicalis) found dead in Santa Catarina state, southern Brazil, presented with disseminated verminous pneumonia due to Parafilaroides sp. A concomitant infection with two different gammaherpesviruses was identified by PCR in different tissues; one of them possibly a novel species (tentatively named Otariid herpesvirus 7). Sarcocystis sp. DNA was identified molecularly in skeletal muscle samples with intrasarcoplasmic bradyzoites and no apparent tissue response. All analyzed samples (mandibular, laryngeal, tracheal, and mesenteric lymph nodes, and lung) were PCR-negative for Brucella spp. The most likely cause of death was severe pulmonary parafilaroidiasis. The pathogenic role of the gammaherpesviruses in several of the tissues was not evident. This study describes the pathogenicity of Parafilaroides sp. in a subantarctic fur seal, widens the host range of herpesvirus in pinnipeds, and reports the first molecular identification of Sarcocystis sp. in this species. Resumo Um lobo-marinho-subantártico (Arctocephalus tropicalis) juvenil foi achado morto no Estado de Santa Catarina, sul do Brasil, apresentando pneumonia parasitária disseminada por Parafilaroides sp. Infecção concomitante por dois gammaherpesvírus diferentes foi identificada pela PCR em diversos tecidos, um desses herpesvírus possivelmente uma nova espécie (denominada provisoriamente Otariid herpesvirus 7). DNA de Sarcocystis sp. foi identificado molecularmente em amostras de músculo esquelético que apresentavam bradizoítos intra-sarcoplasmáticos sem aparente resposta tecidual. Todas as amostras analisadas (linfonodo mandibular, laríngeo, traqueal e mesentérico, e pulmão) pela PCR para Brucella spp. foram negativas. A causa mais provável da morte do animal foi parafilaroidose pulmonar severa. O papel patogénico dos gammaherpesvírus em vários tecidos não foi evidente. Este estudo descreve a patogenicidade de Parafilaroides sp. em um lobo-marinho-subantártico, amplia a variedade de hospedeiros de herpesvírus em pinípedes e reporta a primeira identificação molecular de Sarcocystis sp. para essa espécie.
- Published
- 2019
5. Novel herpesviruses in riverine and marine cetaceans from South America.
- Author
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Sacristán, Carlos, Esperón, Fernando, Ewbank, Ana Carolina, Díaz-Delgado, Josué, Ferreira-Machado, Eduardo, Costa-Silva, Samira, Sánchez-Sarmiento, Angélica María, Groch, Kátia R., Neves, Elena, Pereira Dutra, Gustavo Henrique, Gravena, Waleska, Ferreira Da Silva, Vera Maria, Marcondes, Milton C.C., Castaldo Colosio, Adriana, Cremer, Marta J., Carvalho, Vitor L., O. Meirelles, Ana Carolina, Marigo, Juliana, and Catão-Dias, José Luiz
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HERPESVIRUSES , *ORAL mucosa , *ATLANTIC spotted dolphin , *CETACEAN populations , *POLYMERASE chain reaction - Abstract
Graphical abstract We detected four new cases of herpesvirus infection in skin and oral mucosa of an Atlantic spotted dolphin, a Guiana dolphin, a dwarf sperm whale, and a Bolivian river dolphin from Brazil. Highlights • This is the largest herpesvirus survey in South American cetaceans. • Identification of alpha- and gammaherpesvirus potentially related to skin lesions. • Novel species: Delphinid herpesvirus (HV) 10 , Kogiid HV 2 and 3 , and Iniid HV 1. • Iniid HV 1 phylogeny could be due to viral coevolution with an isolated host. • Expansion of cetacean herpesvirus host range, and the first in riverine dolphins. Abstract Herpesvirus (HV) infections in cetaceans are frequently associated with skin and mucosal lesions. Although HV infections have been reported worldwide, their occurrence in southern Atlantic marine mammals is still poorly understood. We tested skin, oral and genital mucosal beta-actin PCR-positive samples from 109 free-ranging Brazilian cetaceans using a universal herpesvirus DNA polymerase PCR. Herpesvirus-positive skin samples from a Guiana dolphin (Sotalia guianensis), a dwarf sperm whale (Kogia sima), a Bolivian river dolphin (Inia boliviensis), and a lingual sample from an Atlantic spotted dolphin (Stenella frontalis) were histologically evaluated. Additional tissue samples from these animals were also PCR-positive for HV, including a novel sequence obtained from the dwarf sperm whale's stomach and mesenteric lymph node. Four novel HV species were detected in the Guiana dolphin (one), the dwarf sperm whale (two) and the Bolivian river dolphin (one). The cutaneous lesions (marked, focally extensive, chronic proliferative dermatitis) of the Guiana dolphin and the Bolivian river dolphin were similar to previous HV reports in cetaceans, despite the absence of intranuclear inclusion bodies. This is the largest HV survey in South American cetaceans and the first detection of HV infection in riverine dolphins worldwide. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
6. Short-Finned Pilot Whale Strandings Associated with Pilot Whale Morbillivirus, Brazil
- Author
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Samira Costa-Silva, Carlos Sacristán, Rodrigo M. Soares, Vitor L. Carvalho, Pedro V. Castilho, Marta J. Cremer, Ana Carolina Ewbank, Arícia Duarte-Benvenuto, Thalita Faita, Pedro E. Navas-Suárez, Jenyffer V. Vieira, Letícia G. Pereira, Carolina F. Alves, Gabriela C. Souza, Giulia G. Lemos, Natália Silvestre-Perez, José L. Catão-Dias, Lara B. Keid, Fundação de Amparo à Pesquisa do Estado de São Paulo, Sacristán, Carlos, Cremer, Marta J., Ewbank, Ana Carolina, Duarte-Benvenuto, Arícia, Faita, Thalita, Navas-Suárez, Pedro E., and Silvestre-Perez, Natália
- Subjects
Microbiology (medical) ,Pilot whale ,Infectious Diseases ,Morbillivirus ,Epidemiology ,Viruses ,Stranding ,Herpesvirus ,Cetacean ,South America ,PARAMYXOVIRIDAE ,Brazil - Abstract
4 Pág., Cetacean morbillivirus (CeMV) causes illness and death in cetaceans worldwide; the CeMV strains circulating in the Southern Hemisphere are poorly known. We detected a pilot whale CeMV strain in 3 short-finned pilot whales (Globicephala macrorhynchus) stranded in Brazil during July-October 2020. Our results confirm this virus circulates in this species., The Coordination for the Improvement of Higher Education Personnel (CAPES), National Council for Technological and Scientific Development (CNPq), and São Paulo Research Foundation (FAPESP) provided financial support. S.C.S. and A.C.E. received PhD fellowships by FAPESP (process nos. 2020/12434-9 and 2016/20956-0). L.B.K. received financial support from FAPESP (no. 2020/12434-9). J.L.C.-D., L.B.K. and M.J.C. are recipients of research productivity fellowships from CNPq (nos.304999-18, 315619/2021-0, and 313577/2020-0, respectively),C.S. is a recipient of a Juan de la Cierva incorporación fellowship (no. IJC2020-046019-I) and received a postdoctoral grant by FAPESP (no. 2018/25069-7)
- Published
- 2023
- Full Text
- View/download PDF
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