1. The influence of storage temperature on fracture behavior of cryopreserved teeth—An in vitro study
- Author
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Dirk Nolte, Johannes Angermair, Robert Linsenmann, and Karl‐Heinz Kunzelmann
- Subjects
Adult ,Male ,Molar ,Materials science ,Adolescent ,critical loading ,cryopreservation of teeth ,In Vitro Techniques ,Cryopreservation ,Specimen Handling ,Tooth Fractures ,Young Adult ,stomatognathic system ,Dentin ,medicine ,Humans ,Cyclic loading ,In vitro study ,General Dentistry ,Orthodontics ,Mechanical load ,Critical load ,Enamel paint ,Original Articles ,Organ Preservation ,lcsh:RK1-715 ,stomatognathic diseases ,medicine.anatomical_structure ,fracture ,storage temperature ,lcsh:Dentistry ,visual_art ,visual_art.visual_art_medium ,Original Article ,Female ,Tooth - Abstract
Objectives Cryopreservation is discussed as a viable method of preserving teeth for determined autogenous tooth transplantation. Unchanged physical properties of hard tooth tissues are crucial for functional healing. Due to different thermal expansion coefficients of enamel and dentin or the crystallization process, the freezing process may lead to crack formation, which could adversely impact the long‐term prognosis of the teeth. Material and methods Twenty third molars (n = 20) were frozen slowly using a conservative cryopreservation protocol and stored at −80°C (group 1) and −196°C (group 2). After a storage time of 2 weeks, the samples were thawed to a temperature of +36°C and embedded in polymethyl methacrylate blocks. Cyclic loading was carried out using a spherical steel test specimen with 50,000 mechanical load cycles, followed by load to failure testing for determination of critical load. Results No significant difference in the first load drop could be detected during the load to failure test under different storage conditions. The values until fracture correlated very closely in contralateral tooth pairs, which emphasizes the importance of crown geometry in load to failure tests. Conclusions Conclusions: Cryopreservation, specifically the storage temperature, does not appear to have a significant effect on the physical properties of tooth transplants.
- Published
- 2020