1. Detection of circulating tumor cells in colorectal and gastric cancer using a multiplex PCR assay.
- Author
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Vaiopoulos AG, Kostakis ID, Gkioka E, Athanasoula KCh, Pikoulis E, Papalambros A, Christopoulos P, Gogas H, Kouraklis G, and Koutsilieris M
- Subjects
- Adenocarcinoma, Mucinous blood, Adenocarcinoma, Mucinous genetics, Adult, Aged, Aged, 80 and over, Colorectal Neoplasms blood, Colorectal Neoplasms genetics, Female, Humans, Male, Middle Aged, Neoplasm Grading, Neoplasm Metastasis, Neoplasm Staging, Prognosis, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Stomach Neoplasms blood, Stomach Neoplasms genetics, Adenocarcinoma, Mucinous secondary, Biomarkers, Tumor genetics, Colorectal Neoplasms pathology, Multiplex Polymerase Chain Reaction methods, Neoplastic Cells, Circulating pathology, Stomach Neoplasms pathology
- Abstract
Aim: The aim of this study was the development of a multiplex-PCR assay for the detection of circulating tumor cells in patients with colorectal and gastric cancer., Patients and Methods: Peripheral blood samples were collected from 81 patients with colorectal cancer, 16 with gastric cancer and 38 healthy blood donors, as controls. The samples were processed for RNA extraction and cDNA synthesis and were subsequently analyzed for the expression of cytokeratin 19 (CK19), cytokeratin 20(CK20) and epidermal growth factor receptor (EGFR) with multiplex PCR., Results: Statistical analysis revealed that the combination of CK19 and CK20 could be useful in the exclusion of colorectal cancer, as well as the diagnosis and exclusion of gastric cancer. Furthermore, the expression of EGFR was correlated with the presence of systemic disease in patients with colorectal cancer., Conclusion: Multiplex-PCR-based detection of circulating tumor cells could serve as a useful tool for the diagnosis, and monitoring of patients with colorectal and gastric cancer., (Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
- Published
- 2014