3 results on '"Cell differentiations"'
Search Results
2. Stem cell function is conserved during shortterm storage of cultured epidermal cell sheets at 12°C
- Author
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Kim Alexander Tønseth, Sjur Reppe, Catherine Jackson, Håkon Ringstad, Tor Paaske Utheim, and Tine Hiorth Schøyen
- Subjects
0301 basic medicine ,Keratinocytes ,Integrins ,Time Factors ,Apoptosis ,Stem cells ,Apoptoses ,Epithelium ,0302 clinical medicine ,Spectrum Analysis Techniques ,Animal Cells ,Medicine and Health Sciences ,Incubation ,Cells, Cultured ,Confluency ,Multidisciplinary ,medicine.diagnostic_test ,Cell Death ,Stem Cells ,Cell Cycle ,Cell Differentiation ,Flow Cytometry ,Extracellular Matrix ,Cold Temperature ,Cell Processes ,Spectrophotometry ,030220 oncology & carcinogenesis ,Medicine ,Female ,Cytophotometry ,Stem cell ,Cellular Structures and Organelles ,Cellular Types ,Anatomy ,Research Article ,Sterility ,Cell Survival ,Science ,Cell Enumeration Techniques ,Biology ,Research and Analysis Methods ,Flow cytometry ,Andrology ,Viable cell counting ,03 medical and health sciences ,Young Adult ,Viable Cell Counting ,medicine ,Cell Adhesion ,Humans ,VDP::Medisinske Fag: 700 ,Cell Proliferation ,Cryopreservation ,Biology and Life Sciences ,Epithelial Cells ,Cell Biology ,Transplantation ,VDP::Medical disciplines: 700 ,030104 developmental biology ,Biological Tissue ,Vacuoles ,Epidermis ,Function (biology) ,Developmental Biology ,Cell differentiations - Abstract
Transplantation of cultured epidermal cell sheets (CES) can be life-saving for patients with large area burns. CES have also been successfully used to regenerate eye and urethral epithelia in animal models. Short-term storage aims to extend the transplantation window, offers flexibility in timing surgery and allows testing of CES quality, phenotype and sterility. This study investigated extended CES storage and explored the effect of additional re-incubation recovery time following storage. The proliferative quality of stored confluent versus pre-confluent CES was also investigated using functional testing. CES were stored at 12˚C and results compared to non-stored control CES. Investigation of timepoints during 15 days storage revealed that viability began to deteriorate by day 11 and was associated with increased lactate in the storage medium. The percentage of apoptotic cells also significantly increased by day 11. Flow cytometry analysis of integrinβ1 expression and cell size indicated best retention of stem cells at 7 days of storage. Functional testing of pre-confluent and confluent cells following 7 days storage showed that pre-confluent cells responded well to 1-day re-incubation after storage; they became highly prolific, increasing in number by ~67%. Conversely, proliferation in stored confluent cells declined by ~50% with 1-day reincubation. Pre-confluent stored CES also had far superior stem cell colony forming efficiency (CFE) performance compared to the confluent group. Re-incubation improved CFE in both groups, but the pre-confluent group again out-performed the confluent group with significantly more colonies. In conclusion, a maximum storage period of 7 days is recommended. Use of pre-confluent cells and one day recovery incubation greatly improves viability, colony-forming ability and proliferation of cells stored for 7 days at 12˚C. Thus, these recommendations should be considered under culture and storage of high-quality CES for clinical use. The work was supported by funding from South East Norway Regional Health Authority, Norway and by the University of Oslo, Norway. https://www.helse-sorost.no/south-easternnorway-regional-health-authority.
- Published
- 2020
3. Effects of mineral trioxide aggregate, BiodentineTM and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth
- Author
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Bruno das Neves Cavalcanti, Leopoldo Cosme-Silva, Leandro Borges Araújo, Ana Paula Morais Fernandes, Thais Marchini de Oliveira, João Eduardo Gomes Filho, Vivien Thiemy Sakai, Univ Fed Alfenas, Universidade de São Paulo (USP), Univ Iowa, and Universidade Estadual Paulista (Unesp)
- Subjects
0301 basic medicine ,Time Factors ,Cellular differentiation ,Dental pulp capping ,Stem cells ,Extracellular matrix ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Movement ,Materials Testing ,Dentin ,Aluminum Compounds ,Cells, Cultured ,Extracellular Matrix Proteins ,Calcium hydroxide ,Chemistry ,Reverse Transcriptase Polymerase Chain Reaction ,Glyceraldehyde-3-Phosphate Dehydrogenases ,Cell Differentiation ,Oxides ,Drug Combinations ,medicine.anatomical_structure ,Stem cell ,Mineral trioxide aggregate ,Cell Survival ,Andrology ,Calcium Hydroxide ,Biomaterials ,03 medical and health sciences ,medicine ,Humans ,MTT assay ,Viability assay ,Tooth, Deciduous ,General Dentistry ,Cell Proliferation ,Analysis of Variance ,Silicates ,Reproducibility of Results ,DENTE DECÍDUO ,030206 dentistry ,Original Articles ,Calcium Compounds ,Phosphoproteins ,lcsh:RK1-715 ,030104 developmental biology ,lcsh:Dentistry ,Vital pulp therapy ,Pulp Capping and Pulpectomy Agents ,Cell differentiations - Abstract
Made available in DSpace on 2020-12-11T06:51:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01. Added 1 bitstream(s) on 2021-07-15T15:25:15Z : No. of bitstreams: 1 S1678-77572018000100417.pdf: 559476 bytes, checksum: 4090a8c9b3d7d4218b62480babfe0df9 (MD5) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Objective: The aim of the study was to evaluate the effects of the capping materials mineral trioxide aggregate (MTA), calcium hydroxide (CH) and Biodentine (TM) (BD) on stem cells from human exfoliated deciduous teeth (SHED) in vitro. Material and Methods: SHED were cultured for 1 - 7 days in medium conditioned by incubation with MTA, BD or CH (1 mg/mL), and tested for viability (MTT assay) and proliferation (SRB assay). Also, the migration of serum-starved SHED towards conditioned media was assayed in companion plates, with 8 mm-pore-sized membranes, for 24 h. Gene expression of dentin matrix protein-1 (DMP-1) was evaluated by reverse-transcription polymerase chain reaction. Regular culture medium with 10% FBS (without conditioning) and culture medium supplemented with 20% FBS were used as controls. Results: MTA, CH and BD conditioned media maintained cell viability and allowed continuous SHED proliferation, with CH conditioned medium causing the highest positive effect on proliferation at the end of the treatment period (compared with BD and MTA) (p
- Published
- 2018
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