1. Industrial-scale fabrication of an osteogenic and antibacterial PLA/silver-loaded calcium phosphate composite with significantly reduced cytotoxicity.
- Author
-
Cai S, Pourdeyhimi B, and Loboa EG
- Subjects
- Adipose Tissue cytology, Cell Differentiation drug effects, Humans, Stem Cells cytology, Tissue Scaffolds chemistry, Adipose Tissue metabolism, Anti-Bacterial Agents chemical synthesis, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents pharmacology, Calcium Phosphates chemistry, Calcium Phosphates pharmacology, Escherichia coli growth & development, Osteogenesis drug effects, Polyesters chemical synthesis, Polyesters chemistry, Polyesters pharmacology, Silver chemistry, Silver pharmacology, Staphylococcus aureus growth & development, Stem Cells metabolism
- Abstract
In this study, we report an industrial-scale fabrication method of a multifunctional polymer composite as a scaffold material for bone tissue engineering. This study successfully demonstrated the potential of applying industrial polymer processing technologies to produce specially functionalized tissue engineering scaffolds. With the inclusion of a newly synthesized multifunctional additive, silver-doped-calcium phosphate (silver-CaP), the composite material exhibited excellent osteogenic inducibility of human adipose-derived stem cells (hASC) and satisfactory antibacterial efficacy against Escherichia coli and Staphylococcus aureus. Also, relative to previously reported methods of direct loading silver particles into polymeric materials, our composite exhibited significantly reduced silver associated cytotoxicity. The enhanced biocompatibility could be a significant advantage for materials to be used for regenerative medicine applications where clinical safety is a major consideration. The impact of different silver loading methodologies on hASC' osteogenic differentiation was also studied. Overall, the results of this study indicate a promising alternative approach to produce multifunctional scaffolds at industrial-scale with higher throughput, lower cost, and enhanced reproducibility. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 900-910, 2019., (© 2018 Wiley Periodicals, Inc.)
- Published
- 2019
- Full Text
- View/download PDF