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The objective of this research was to evaluate activation of the ileal brake in broiler chickens using diets containing semi-purified wheat (WS; rapidly and highly digested) and pea (PS; slowly and poorly digested) starch. Diets were formulated to contain six WS:PS ratios (100:0, 80:20, 60:40, 40:60, 20:80, 0:100) and each starch ratio was fed to 236 Ross 308 male broilers housed in 4 litter floor pens. At 28 d of age, the effect of PS concentration was assessed on starch digestion, digestive tract morphology, and digesta pH and short-chain fatty acid (SCFA) concentration. Glucagon-like peptide-1 (GLP-1) and peptide tyrosine-tyrosine (PYY) status were assessed in serum (ELISA) and via gene expression in jejunal and ileal tissue (proglucagon for GLP-1). Data were analyzed using regression analyses, and significance was accepted at P ≤ 0.05. Increasing dietary PS resulted in reduced starch digestibility in the small intestine, but had no effect in the colon. Crop content pH responded quadratically to PS level with an estimated minimum at 55% PS. Total SCFA increased linearly in the crop with PS level, but changed in a quadratic fashion in the ileum (estimated maximum at 62% PS). Ceacal SCFA concentrations were highest for the 80 and 100% PS levels. The relative empty weight (crop, small intestine, colon), length (small intestine) and content (crop jejunum, Ileum) of digestive tract sections increased linearly with increasing PS concentration. Dietary treatment did not affect serum GLP-1 or PYY or small intestine transcript abundance. In conclusion, feeding PS increased the presence of L-cell activators (starch, SCFA) and increased trophic development and content of the digestive tract, suggestive of L-cell activation. However, no direct evidence of ileal brake activation was found by measuring venous blood levels of GLP-1 or PYY or corresponding gene expression in small intestine tissue., Competing Interests: We have the following competing interests: This study was funded in part by Aviagen North America, Sofina Foods Inc., Prairie Pride Natural Foods Ltd., Chicken Farmers of Saskatchewan, Canadian Poultry Research Council, Poultry Industry Council (Canada), Saskatchewan Broiler Hatching Egg Producer's Marketing Board, Saskatchewan Egg Producers and Saskatchewan Turkey Producers' Marketing Board. There are no patents, products in development or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.

Dietary starch with lower rate and extent of digestion improves broiler feed efficiency, but previous results might have been confounded by non-starch components of the grains. Therefore, the objective of this research was to study the effects of starch digestion on broilers using semi-purified starch. Semi-purified wheat (WS, rapidly digested) and pea (PS, slowly digested) starch were combined to create 6 WS:PS ratios (100:0, 80:20, 60:40, 40:60, 20:80, and 0:100) in starter, grower and finisher diets. Each treatment was fed to Ross 308 male (2,124) and female (2,376) broilers housed in 72 L floor pens from 0 to 31 d of age to measure performance and meat yield relative to live weight. On day 33, the effects of diet on 8 h feed withdrawal was assessed in 20 males per treatment. Data were analyzed with ANOVA and linear and quadratic regression analyses using SAS 9.4. Significance was accepted at P ≤ 0.050. Body weight gain declined linearly with increasing PS. Male feed intake decreased with increasing PS, but PS did not affect female feed intake. Mortality corrected gain:feed ratio was quadratically influenced by diet (estimated maximum at 25% PS). Breast meat increased linearly with PS, while fat pad and breast and thigh skin decreased linearly. Quadratic responses were found for thigh meat and whole drum (estimated maximum values at 56 and 54% PS, respectively). Males grew faster, ate more, and had higher mortality than females. They also had heavier pectoralis major, thigh bone, and whole drum, while females had heavier pectoralis minor and more breast and thigh skin. After feed withdrawal, digesta content decreased linearly with time in all sections, except for the crop and duodenum, which declined quadratically. Ileal digesta pH increased linearly with time, while crop and caecal pH decreased for 2 h before steadily increasing. Diet did not affect digestive tract emptying or digesta pH. In conclusion, dietary PS maximized feed efficiency at 25% PS and linearly improved breast meat yield, but did not affect digesta clearance after feed withdrawal., (© 2019 Poultry Science Association Inc.)

The inclusion of starch with a lower rate and extent of digestion has proven to be beneficial in broiler production. However, less is known about its effect on laying hen performance. Therefore, 6 diets were formulated to produce differing ratios (0:100, 20:80, 40:60, 60:40, 80:20, and 100:0) of semi-purified wheat starch (rapidly digested) and pea starch (PS; slowly digested). Each diet was fed to 120 conventionally caged Lohmann LSL lite hens in groups of 12, from 27 to 46 wk of age, and its effects on performance and feather covering were assessed. Data were analyzed by regression analysis, and the significance level was chosen at P ≤ 0.05. Hen-day egg production (HDP) was high (97.05%) and unaffected by PS concentration for 0 to 10 wk of the trial, followed by a quadratic-shaped HDP, with an estimated maximum at 67% PS concentration, for the 10 to 20 wk period of the trial. Overall (0 to 20 wk), HDP increased linearly with PS. No effect on egg weight was found (average weight = 59.6±2.1 g), but eggshells were thickest at the 55% PS concentration. Body weight gain was affected by PS concentration and maximized at 49% PS. Body weight uniformity and mortality were not affected by dietary treatment. Feed intake increased linearly with PS from 102 to 109 g/hen/d, while feed: egg mass ratio was minimal at 26% PS. Using a scale from 1 to 4 per body part (neck, wings, back, vent and breast), only neck feather cover increased linearly with PS, from 3.0 (0% PS) to 3.2/4 (100% PS). However, back and total feather coverage showed a trend (P = 0.054 and P = 0.079 respectively) to increase linearly with PS as well (from 3.7 to 3.9/4 and 15.6 to 16.6/20, respectively). In summary, feeding PS at low to intermediate concentrations improved laying hen performance. Further research should focus on the mechanisms involved in this effect., (© 2019 Poultry Science Association Inc.)